Simple Plex Human Hepcidin Cartridge

Catalog #: SPCKB-PS-000984 Datasheet / COA / SDS

Simple Plex Human Hepcidin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000984
SPCKA-PS-000984
SPCKC-PS-000984

Control Products Available

898483: Simple Plex Control for Human Hepcidin

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human Hepcidin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)

Sensitivity

4.81 pg/mL

Assay Range

9.7 - 37,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Hepcidin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Hepcidin in serum and plasma (EDTA/Heparin)

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	109	106-112
Heparin Plasma (n=4)	100	92-113
Serum (n=4)	109	106-117

Linearity

Samples containing and/or spiked with high concentrations of human Hepcidin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay