Simple Plex Human IFN-beta Cartridge

Catalog #: SPCKB-PS-000934 Datasheet / COA / SDS

Simple Plex Human IFN-beta assay kit contains cartridge, sample diluent SD10, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000934
SPCKA-PS-000934
SPCKC-PS-000934
ST01B-PS-000934

Control Products Available

898790: Simple Plex Control for Human IFN-beta

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IFN-beta Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.03 pg/mL

Assay Range

1.2 - 1,804 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma), 6.2 - 3,760 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IFN-beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IFN-beta in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	28
Mean (pg/mL)	13.5	551	11.2	507
Standard Deviation	1.27	45.9	1.38	57.9
CV%	9.4	8.3	12.3	11.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	97	91-104
EDTA Plasma (n=4)	92	84-101
Heparin Plasma (n=4)	80	76-86
Serum (n=4)	95	83-106

Linearity

Samples containing and/or spiked with high concentrations of human IFN-beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human IFN-beta Ella Assay Standard Curve - Serum/plasma with Sample Diluent SD10

Scientific Data

N/A

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Human IFN-beta Ella Assay Standard Curve - CCS with Sample Diluent SD13

N/A

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Human IFN-beta Ella Assay Standard Curve - Serum/Plasma with Sample Diluent SD10

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name:

Interferon beta

Entrez Gene IDs:

3456 (Human); 15977 (Mouse); 24481 (Rat)

Alternate Names:

Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956