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Simple Plex Human IL-8 Cartridge

Catalog #: SPCKB-PS-000230
1 Citation Datasheet / COA / SDS
Simple Plex Human IL-8/CXCL8 assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)
Catalog # Availability Size / Price Qty
SPCKB-PS-000230
SPCKA-PS-000230
SPCKC-PS-000230
ST01B-PS-000230
Control Products Available
894950: Simple Plex Control for Human IL-8/CXCL8
Human IL-8 Simple Plex Assay
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Citations (1)
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IL-8 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity
0.08 pg/mL
Assay Range
0.2 - 1,804 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Specificity
Natural and recombinant human IL-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-8/CXCL8 (IL-8) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=5) 89 75-102
Heparin Plasma (n=5) 95 88-102
Serum (n=3) 92 88-97

Linearity

Samples containing and/or spiked with high concentrations of human IL-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human IL-8 Ella Assay Linearity

Scientific Data

N/A Human IL-8 Simple Plex Assay Standard Curve View Larger

Human IL-8 Ella Assay Standard Curve

Product Datasheets

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Product Datasheet
COA
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Entrez Gene IDs:
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names:
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

Citation for Simple Plex Human IL-8 Cartridge

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Torquetenovirus in pregnancy: Correlation with vaginal microbiome, metabolome and pro-inflammatory cytokines
    Authors: S Morselli, C Foschi, L Laghi, S Zagonari, G Patuelli, T Camboni, C Ceccarani, C Consolandi, ME Djusse, MF Pedna, A Marangoni, M Severgnini, V Sambri
    Frontiers in Microbiology, 2022;13(0):998849.
    Species: Human
    Sample Types: Cell Culture Supernatants

FAQs

  1. Are mouse or rat CXCL8/IL-8 products offered?

    • Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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