Home / NF-H / Simple Plex Human NF-H Cartridge

Simple Plex Human NF-H Cartridge

Catalog #: SPCKB-PS-000519
4 Citations Datasheet / COA / SDS
Simple Plex Human NF-H assay kit contains cartridge, sample diluent SD06, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)
Catalog # Availability Size / Price Qty
SPCKB-PS-000519
SPCKA-PS-000519
SPCKC-PS-000519
Control Products Available
898312: Simple Plex Control for Human NF-H
Human NF-H Simple Plex Assay
3 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human NF-H Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (13 uL)
Sensitivity
1.17 pg/mL
Assay Range
7.5 - 28,480 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Specificity
Natural and recombinant human NF-H.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Customers also Viewed

Recombinant Human Megalin/LRP-2 (C3) Protein, CF
Cat # 9578-LR

Product Summary

Simple Plex assay for the detection of human Neurofilament Heavy (NF-H) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 10 9
Mean (pg/mL) 43.4 2206 47.4 2881
Standard Deviation 2.22 81.6 6.59 365
CV% 5.1 3.7 13.9 12.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=3) 98 93-101
EDTA Plasma (n=4) 94 83-108
Heparin Plasma (n=4) 102 99-107
Serum (n=4) 102 82-114

Linearity

Samples containing and/or spiked with high concentrations of human NF-H were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human NF-H Simple Plex Assay Linearity

Scientific Data

N/A Human NF-H Simple Plex Assay Standard Curve View Larger

Human NF-H Simple Plex Assay Standard Curve

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-H

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name:
Neurofilament Protein, Heavy Chain
Entrez Gene IDs:
4744 (Human)
Alternate Names:
200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H

Citations for Simple Plex Human NF-H Cartridge

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Neurofilament as a potential biomarker for spinal muscular atrophy.
    Authors: Darras B, Crawford T, Finkel R, Mercuri E, De Vivo D, Oskoui M, Tizzano E, Ryan M, Muntoni F, Zhao G, Staropoli J, McCampbell A, Petrillo M, Stebbins C, Fradette S, Farwell W, Sumner C
    Ann Clin Transl Neurol, 0;6(5):932-944.
    Species: Human
    Sample Types: Plasma
  2. Plasma Neurofilament Light for Prediction of Disease Progression in Familial Frontotemporal Lobar Degeneration.
    Authors: Rojas J, Wang P, Staffaroni A, Heller C, Cobigo Y, Wolf A, Goh S, Ljubenkov P, Heuer H, Fong J, Taylor J, Veras E, Song L, Jeromin A, Hanlon D, Yu L, Khinikar A, Sivasankaran R, Kieloch A, Valentin M, Karydas A, Mitic L, Pearlman R, Kornak J, Kramer J, Miller B, Kantarci K, Knopman D, Graff-Radford N, Petrucelli L, Rademakers R, Irwin D, Grossman M, Ramos E, Coppola G, Mendez M, Bordelon Y, Dickerson B, Ghoshal N, Huey E, Mackenzie I, Appleby B, Domoto-Reilly K, Hsiung G, Toga A, Weintraub S, Kaufer D, Kerwin D, Litvan I, Onyike C, Pantelyat A, Roberson E, Tartaglia M, Foroud T, Chen W, Czerkowicz J, Graham D, van Swieten J, Borroni B, Sanchez-Valle R, Moreno F, Laforce R, Graff C, Synofzik M, Galimberti D, Rowe J, Masellis M, Finger E, Vandenberghe R, de Mendonca A, Tagliavini F, Santana I, Ducharme S, Butler C, Gerhard A, Levin J, Danek A, Otto M, Sorbi S, Cash D, Convery R, Bocchetta M, Foiani M, Greaves C, Peakman G, Russell L, Swift I, Todd E, Rohrer J, Boeve B, Rosen H, Boxer A
    Neurology, 0;96(18):e2296-e2312.
    Species: Human
    Sample Types: CSF
  3. Implications of circulating neurofilamentsfor spinal muscular atrophytreatment early in life: A case series.
    Authors: Alves C, Petrillo M, Spellman R, Garner R, Zhang R, Kiefer M, Simeone S, Sohn J, Eichelberger E, Rodrigues E, Arruda E, Townsend E, Farwell W, Swoboda K
    Mol Ther Methods Clin Dev, 0;23(0):524-538.
    Species: Human
    Sample Types: Serum
  4. Comparison of CSF and serum neurofilament light and heavy chain as differential diagnostic biomarkers for ALS.
    Authors: Halbgebauer S, Steinacker P, Verde F, Weishaupt J, Oeckl P, von Arnim C, Dorst J, Feneberg E, Mayer B, Rosenbohm A, Silani V, Ludolph A, Otto M
    Oncogene, 0;93(1):68-74.
    Species: Human
    Sample Types: CSF

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs
Loading...

Reviews for Simple Plex Human NF-H Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human NF-H Cartridge and earn rewards!

Have you used Simple Plex Human NF-H Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review