Simple Plex Human Pro-GRP Cartridge

Catalog #: SPCKB-PS-000922 Datasheet / COA / SDS

Simple Plex Human Pro-GRP/Gastrin-releasing Peptide/Bombesin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000922
SPCKA-PS-000922
SPCKC-PS-000922

Control Products Available

898716: Simple Plex Control for Human Pro-GRP

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All Gastrin-releasing Peptide/Bombesin Simple Plex Assays

Product Details

Supplemental Products

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human Pro-GRP Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.53 pg/mL

Assay Range

2.6 - 4,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Pro-Gastrin-releasing Peptide .

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Pro-Gastrin-releasing Peptide in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	5	5
Mean (pg/mL)	27.2	1566	27.1	1561
Standard Deviation	2.18	105	2.94	129
CV%	8	6.7	10.9	8.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=3)	92	88-99
Heparin Plasma (n=3)	94	90-96
Serum (n=3)	101	93-107

Linearity

Samples containing and/or spiked with high concentrations of human Pro-Gastrin-releasing Peptide were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay