Simple Plex Human Procalcitonin Cartridge

Catalog #: SPCKB-PS-001144 Datasheet / COA / SDS

Simple Plex Human Procalcitonin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001144
SPCKA-PS-001144
SPCKC-PS-001144

Control Products Available

898497: Simple Plex Control for Human Procalcitonin

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human Procalcitonin Cartridge Summary

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.79 pg/mL

Assay Range

1.6 - 15,100 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Specificity

Natural and recombinant human Procalcitonin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Procalcitonin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	18	17
Mean (pg/mL)	81.2	4828	83.3	4876
Standard Deviation	5.15	301	4.69	321
CV%	6.3	6.2	5.6	6.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=3)	109	106-113
EDTA Plasma (n=4)	105	97-111
Heparin Plasma (n=4)	103	98-111
Serum (n=4)	105	96-110

Linearity

Samples containing and/or spiked with high concentrations of human Procalcitonin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay