Simple Plex Human TRAIL/TNFSF10 Cartridge

Catalog #: SPCKB-PS-000536 Datasheet / COA / SDS

Simple Plex Human TRAIL/TNFSF10 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000536
SPCKA-PS-000536
SPCKC-PS-000536

Control Products Available

898276: Simple Plex Control for Human TRAIL/TNFSF10

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human TRAIL/TNFSF10 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

2.54 pg/mL

Assay Range

2.6 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human TRAIL.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human TRAIL in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	22
Mean (pg/mL)	59.6	2519	53.4	2654
Standard Deviation	3.9	87.8	3.43	145
CV%	6.5	3.5	6.4	5.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	111	104-119
EDTA Plasma (n=4)	82	77-89
Heparin Plasma (n=4)	81	74-85
Serum (n=4)	78	73-86

Linearity

Samples containing and/or spiked with high concentrations of human TRAIL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human TRAIL Simple Plex Assay Standard Curve

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Human TRAIL Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TRAIL/TNFSF10

TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.

Long Name:

TNF-related Apoptosis-inducing Ligand

Entrez Gene IDs:

8743 (Human); 22035 (Mouse); 246775 (Rat)

Alternate Names:

Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10