Simple Plex Human TREM-1 Cartridge

Catalog #: SPCKB-PS-001020 Datasheet / COA / SDS

Simple Plex Human TREM-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001020
SPCKA-PS-001020
SPCKC-PS-001020

Control Products Available

898622: Simple Plex Control for Human TREM-1

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human TREM-1 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

0.73 pg/mL

Assay Range

4.2 - 40,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Specificity

Natural and recombinant human TREM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Triggering Receptor Expressed on Myeloid cells (TREM-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	23
Mean (pg/mL)	216	11034	220	11170
Standard Deviation	17.9	1013	20.1	673
CV%	8.3	9.2	9.1	6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=3)	119	115-123
Urine (n=5)	118	113-125

Linearity

Samples containing and/or spiked with high concentrations of human TREM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human TREM-1 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM-1

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Long Name:

Triggering Receptor Expressed on Myeloid Cells 1

Entrez Gene IDs:

54210 (Human); 58217 (Mouse)

Alternate Names:

CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1