Simple Plex Human VEGF-B Cartridge

Catalog #: SPCKB-PS-000539 Datasheet / COA / SDS

Simple Plex Human VEGF-B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000539
SPCKA-PS-000539
SPCKC-PS-000539

Control Products Available

898422: Simple Plex Control for Human VEGF-B

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human VEGF-B Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

4.49 pg/mL

Assay Range

10.1 - 15,384 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human VEGF-B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human VEGF-B in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	21
Mean (pg/mL)	208	10568	206	10533
Standard Deviation	10	759	17.6	1312
CV%	4.8	7.2	8.5	12.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	101	95-109
Heparin Plasma (n=4)	98	90-104
Serum (n=4)	100	95-103

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human VEGF-B Simple Plex Assay Standard Curve

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Human VEGF-B Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.

Long Name:

Vascular Endothelial Growth Factor B

Entrez Gene IDs:

7423 (Human); 22339 (Mouse)

Alternate Names:

vascular endothelial growth factor B; VEGFB; VEGF-B; VEGF-related factor; VRFVEGFL