Simple Plex Human VEGF-C Cartridge

Name: Simple Plex Human VEGF-C Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000224
Price: 830 USD

Catalog #: SPCKB-PS-000224 Datasheet / COA / SDS

Simple Plex Human VEGF-C assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer

Discontinued Product

SPCKB-PS-000224 has been discontinued.

View all VEGF-C products.

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All VEGF-C Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human VEGF-C Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL)

Sensitivity

6.9 pg/mL

Assay Range

32.6 - 49,690 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Specificity

Natural and recombinant human VEGF-C.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human VEGF-C in serum and platelet-poor plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	19
Mean (pg/mL)	149	7683	151	7918
Standard Deviation	13.7	531	13.4	727
CV%	9.2	6.9	8.8	9.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Platelet-poor EDTA Plasma (n=4)	111	92-122
Platelet-poor Heparin Plasma (n=4)	111	90-123

Linearity

Samples containing and/or spiked with high concentrations of human VEGF-C were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human VEGF-C Simple Plex Assay Standard Curve

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Human VEGF-C Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Long Name:

Vascular Endothelial Growth Factor C

Entrez Gene IDs:

7424 (Human); 22341 (Mouse)

Alternate Names:

Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM