Simple Plex Mouse CXCL1/KC Cartridge

Catalog #: SPCKB-MP-003558 Datasheet / COA / SDS

Simple Plex Mouse CXCL1/GRO alpha/KC/CINC-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-003558
SPCKA-MP-003558
SPCKC-MP-003558

Control Products Available

896166: Simple Plex Control for Mouse CXCL1/GRO alpha/KC/CINC-1

Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Mouse Plasma/Serum.

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All CXCL1/GRO alpha/KC/CINC-1 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse CXCL1/KC Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.1 pg/mL

Assay Range

0.5 - 1,810 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse KC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse KC in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	99	98
Mean (pg/mL)	9.21	396	12	522
Standard Deviation	0.752	24.1	1.11	45
CV%	8.2	6.1	9.3	8.6

Recovery

Recovery was not necessary as KC shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of mouse KC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Mouse CXCL1/KC Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Entrez Gene IDs:

2919 (Human); 14825 (Mouse); 81503 (Rat)

Alternate Names:

CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1