Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

Catalog #: SPCKB-MP-001485 Datasheet / COA / SDS

Simple Plex Mouse CXCL10/IP-10/CRG-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-001485
SPCKA-MP-001485
SPCKC-MP-001485

Control Products Available

898559: Simple Plex Control for Mouse CXCL10/IP-10/CRG-2

Simple Plex Cartridge Kit for 72 samples, 1 analyte for use with Mouse Plasma/Serum.

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All CXCL10/IP-10/CRG-2 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)

Sensitivity

1.37 pg/mL

Assay Range

3.9 - 6,000 pg/mL (Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Specificity

Natural and recombinant mouse IP-10.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse IP-10 in cell serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	14	15
Mean (pg/mL)	35.1	1918	36.1	1825
Standard Deviation	1.27	124	2.78	155
CV%	3.6	6.5	7.7	8.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=4)	113	109-119
Mouse Heparin Plasma (n=4)	106	95-114
Mouse Serum (n=4)	111	103-117

Linearity

Samples containing and/or spiked with high concentrations of mouse IP-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Mouse CXCL10/IP-10/CRG-2 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Entrez Gene IDs:

3627 (Human); 15945 (Mouse)

Alternate Names:

C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10