Simple Plex Mouse IL-7 Cartridge

Catalog #: SPCKB-MP-004018 Datasheet / COA / SDS
Simple Plex Mouse IL-7 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-MP-004018
SPCKA-MP-004018
SPCKC-MP-004018
Control Products Available
Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Mouse Plasma/Serum.
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Simple Plex Mouse IL-7 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
2.1 pg/mL
Assay Range
20.9 - 31,848 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-7.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse IL-7 in serum and plasma (EDTA/Heparin).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 110 98-120
Heparin Plasma (n=4) 117 115-120
Serum (n=4) 113 109-116

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Mouse IL-7 Ella Assay Linearity

Scientific Data

Mouse IL-7 Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Long Name:
Interleukin 7
Entrez Gene IDs:
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names:
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

FAQs

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