Simple Plex Mouse/Rat FGF-21 Cartridge

Catalog #: SPCKB-MP-006323 Datasheet / COA / SDS

Simple Plex Mouse/Rat FGF-21 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-006323
SPCKA-MP-006323
SPCKC-MP-006323
SPCKA-MP-006323-000466
SPCKC-MP-006323-000466

Control Products Available

897008-SP: Simple Plex Control for Mouse/Rat FGF-21

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat FGF-21 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.7 pg/mL

Assay Range

2.6 - 4,000 pg/mL (Mouse Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

This assay recognizes natural and recombinant mouse/rat FGF-21.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat Fibroblast Growth Factor 21 (FGF-21) in cell culture supernatant (CCS)*, serum, and plasma (EDTA/Heparin)

Recovery

Recovery was not necessary as mouse/rat FGF-21 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat FGF-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Mouse/Rat FGF-21 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-21

FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.

Long Name:

Fibroblast Growth Factor 21

Entrez Gene IDs:

26291 (Human); 56636 (Mouse)

Alternate Names:

FGF21; FGF-21; fibroblast growth factor 21