Simple Plex Mouse/Rat SP-D Cartridge

Catalog #: SPCKB-MP-008605 Datasheet / COA / SDS

Simple Plex Mouse/Rat SP-D assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-008605
SPCKA-MP-008605
SPCKC-MP-008605

Control Products Available

897177: Simple Plex Control for Mouse/Rat SP-D

4 Images

All SP-D Simple Plex Assays

Product Details

Supplemental Products

Reviews

Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat SP-D Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Bronchoalveolar Lavage (10 uL), Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.8 pg/mL

Assay Range

7.1 - 26,909 pg/mL (Bronchoalveolar Lavage Fluid, Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse and rat SP-D.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat Surfactant Protein D (SP-D) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) and Bronchoalveolar Lavage Fluid (BALF)*.
*BALF are validated for mouse samples only.

Recovery

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	92	82-102

Linearity

Samples containing and/or spiked with high concentrations of SP-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

View Larger

Mouse/Rat SP-D Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Long Name:

Surfactant Pulmonary Associated Protein D

Entrez Gene IDs:

6441 (Human); 20390 (Mouse)

Alternate Names:

COLEC7collectin-7; Collectin 7; Collectin-7; Lung surfactant protein D; PSPD; PSP-D; SFTP4; SFTP4pulmonary surfactant-associated protein D; SFTPD; SPD; SP-D; SP-Dpulmonary surfactant apoprotein; surfactant protein D; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs

Reviews for Simple Plex Mouse/Rat SP-D Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Mouse/Rat SP-D Cartridge and earn rewards!

Have you used Simple Plex Mouse/Rat SP-D Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review