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Cultrex Spheroid Invasion Extracellular Matrix

Catalog #: 3500-096-03
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3500-096-03
Spheroid Cell Invasion Assay_3500-096-K
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Citations (9)
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Cultrex Spheroid Invasion Extracellular Matrix Summary

Provides a specialized extracellular matrix that is optimized to drive invasion of cells out of the preformed spheroids as a part of the Cultrex 3-D Culture Spheroid Cell Invasion Assay.

Why Use Cultrex Spheroid Invasion Extracellular Matrix?

The Cultrex Spheroid Invasion Matrix is a proprietary extracellular matrix blend comprised of basement membrane extract, derived from murine EHS sarcoma cells, and collagen I, from bovine extensor tendons. This matrix forms a hydrogel network on which invasive cells can travel out of a preformed spheroid. Cell invasion is visualized microscopically and can be quantified through image analysis software.

Negative for the presence of bacteria and fungi.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Performance tested in Cultrex 3-D Spheroid Cell Invasion Assay - supports invasion of cancer cells (MDA-MB-231 human breast cancer cells or HT-1080 human fibrosarcoma) out of a spheroid over a 96 hour period.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Scientific Data

N/A Spheroid Cell Invasion Assay_3500-096-K View Larger

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay (Catalog # 3500-096-K). Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix (Catalog # 3500-096-01). Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix (Catalog # 3500-096-03). After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Citations for Cultrex Spheroid Invasion Extracellular Matrix

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Stimulation of tumoricidal immunity via bacteriotherapy inhibits glioblastoma relapse
    Authors: Zhang, Y;Xi, K;Fu, Z;Zhang, Y;Cheng, B;Feng, F;Dong, Y;Fang, Z;Zhang, Y;Shen, J;Wang, M;Han, X;Geng, H;Sun, L;Li, X;Chen, C;Jiang, X;Ni, S;
    Nature communications  2024-05-18
  2. Nuclear transport of phosphorylated LanCL2 promotes invadopodia formation and tumor progression of glioblastoma by activating STAT3/Cortactin signaling
    Authors: Zhao, HF;Liu, YS;Wang, J;Wu, CP;Zhou, XM;Cai, LR;Liu, J;Liu, XJ;Xu, YW;Li, WP;Huang, GD;
    Journal of advanced research  2024-03-14
  3. SLC25A32 promotes malignant progression of glioblastoma by activating PI3K-AKT signaling pathway
    Authors: Xue, Z;Wang, J;Wang, Z;Liu, J;Zhao, J;Liu, X;Zhang, Y;Liu, G;Zhao, Z;Li, W;Zhang, Q;Li, X;Huang, B;Wang, X;
    BMC cancer  2023-06-26
  4. Cell senescence-associated genes predict the malignant characteristics of glioblastoma
    Authors: C Tan, Y Wei, X Ding, C Han, Z Sun, C Wang
    Cancer Cell International, 2022-12-16;22(1):411.  2022-12-16
  5. Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis
    Authors: N Mestre-Far, S Guerrero, N Bley, E Rivero, O Coll, E Borràs, E Sabidó, A Indacochea, C Casillas-S, AI Järvelin, B Oliva, A Castello, S Hüttelmaie, F Gebauer
    Nucleic Acids Research, 2022-08-12;0(0):.  2022-08-12
  6. Mutation-specific non-canonical pathway of PTEN as a distinct therapeutic target for glioblastoma
    Authors: SW Choi, Y Lee, K Shin, H Koo, D Kim, JK Sa, HJ Cho, HM Shin, SJ Lee, H Kim, S Chung, J Shin, C Lee, DH Nam
    Cell Death & Disease, 2021-04-07;12(4):374.  2021-04-07
  7. Tumor Treating Fields (TTFields) Hinder Cancer Cell Motility through Regulation of Microtubule and Acting Dynamics
    Authors: T Voloshin, RS Schneiderm, A Volodin, RR Shamir, N Kaynan, E Zeevi, L Koren, A Klein-Gold, R Paz, M Giladi, Z Bomzon, U Weinberg, Y Palti
    Cancers (Basel), 2020-10-17;12(10):.  2020-10-17
  8. DOCK6 promotes chemo- and radioresistance of gastric cancer by modulating WNT/beta-catenin signaling and cancer stem cell traits
    Authors: HC Chi, CY Tsai, CS Wang, HY Yang, CH Lo, WJ Wang, KF Lee, LY Lai, JH Hong, YF Chang, MM Tsai, CT Yeh, CH Wu, CC Hsieh, LH Wang, WJ Chen, KH Lin
    Oncogene, 2020-08-04;0(0):.  2020-08-04
  9. Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms
    Authors: S Featherby, YP Xiao, C Ettelaie, LL Nikitenko, J Greenman, A Maraveyas
    Sci Rep, 2019-04-18;9(1):6272.  2019-04-18

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