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TBARS Parameter Assay Kit

Catalog #: KGE013
45 Citations 3 Reviews Datasheet / COA / SDS
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KGE013
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QC164: Parameter Immunoassay Control Set 752 for TBARS
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Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve
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Product Details
Procedure
Citations (45)
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Reviews (3)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

TBARS Parameter Assay Kit Summary

Format
96-well strip plate
Assay Length
2-3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (150 uL), Cell Lysates (150 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (150 uL)
Sensitivity
0.055 uM
Assay Range
0.0 - 17 uM
Specificity
TBARS in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (µM) 2.57 6.07 9.26 2.39 5.71 8.76
Standard Deviation 0.025 0.078 0.109 0.125 0.242 0.322
CV% 1 1.3 1.2 5.2 4.2 3.7

Recovery

The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 94-110
Cell Lysis Buffer 3 (n=1) 99 96-103
EDTA Plasma (n=4) 91 89-106
Heparin Plasma (n=4) 99 94-108
Serum (n=4) 98 87-105
Urine (n=4) 99 91-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.
Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Linearity

Scientific Data

N/A Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve View Larger

Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
&#9888; WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, TBARS standard, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 150 µL Standards and Samples
  3.   Add 150 µL of standards and samples to each well.
    4. 75 µL TBA Reagent
  4.   Add 75 µL of TBA Reagent to each well.
  5.   Pre-read the optical density of each well using a microplate reader set to 532 nm.
  6.   Incubate the microplate for 2-3 hours at 45-50 °C.
  7.   Read the optical density of each well using a microplate reader set to 532 nm. Subtract the pre-reading from the final reading to correct for the sample's contributions to the final absorption at 532 nm.

Citations for TBARS Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. Acid sphingomyelinase inhibition induces cerebral angiogenesis post-ischemia/reperfusion in an oxidative stress-dependent way and promotes endothelial survival by regulating mitochondrial metabolism
    Authors: Mohamud Yusuf, A;Borbor, M;Hussner, T;Weghs, C;Kaltwasser, B;Pillath-Eilers, M;Walkenfort, B;Kolesnick, R;Gulbins, E;Hermann, DM;Brockmeier, U;
    Cell death & disease
    Species: Mouse
    Sample Types: Plasma, Tissue Homogenates
  2. Delineating the Molecular Mechanisms of Hippocampal Neurotoxicity Induced by Chronic Administration of Synthetic Cannabinoid AB-FUBINACA in Mice
    Authors: Alzu'bi, A;Abu-El-Rub, E;Almahasneh, F;Tahat, L;Athamneh, RY;Khasawneh, R;Alzoubi, H;Ghorab, DS;Almazari, R;Zoubi, MSA;Al-Zoubi, RM;
    Neurotoxicology
    Species: Mouse
    Sample Types: Protein
  3. Rebaudioside D decreases adiposity and hepatic lipid accumulation in a mouse model of obesity
    Authors: Morissette, A;de Wouters d'Oplinter, A;Andre, DM;Lavoie, M;Marcotte, B;Varin, TV;Trottier, J;Pilon, G;Pelletier, M;Cani, PD;Barbier, O;Houde, VP;Marette, A;
    Scientific reports
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Blackcurrant Anthocyanins Improve Blood Lipids and Biomarkers of Inflammation and Oxidative Stress in Healthy Women in Menopause Transition without Changing Body Composition
    Authors: Nosal, BM;Sakaki, JR;Mofrad, MD;Macdonald, Z;Mahoney, KJ;Thornton, SN;Patel, D;Drossman, J;Lee, EC;Chun, OK;
    Biomedicines
    Species: Human
    Sample Types: Plasma
  5. Effect of the Fermented Soy Q-CAN® Product on Biomarkers of Inflammation and Oxidation in Adults with Cardiovascular Risk, and Canonical Correlations between the Inflammation Biomarkers and Blood Lipids
    Authors: Jung, SM;Kaur, A;Amen, RI;Oda, K;Rajaram, S;Sabatè, J;Haddad, EH;
    Nutrients
    Species:  Human
    Sample Types: Plasma
  6. Microvascular Function, Inflammatory Status, and Oxidative Stress in Post-Bariatric Patients with Weight Regain
    Authors: Lopes, KG;de Souza, MDGC;Bouskela, E;Kraemer-Aguiar, LG;
    Nutrients
    Species: Human
    Sample Types: Serum
  7. A Novel Tool to Identify Bactericidal Compounds against Vulnerable Targets in Drug-Tolerant M. tuberculosis found in Caseum
    Authors: JP Sarathy, M Xie, RM Jones, A Chang, P Osiecki, D Weiner, WS Tsao, M Dougher, L Blanc, N Fotouhi, LE Via, CE Barry, I De Vlaminc, DR Sherman, VA Dartois
    MBio, 2023-04-05;14(2):e0059823.
    Species: Rabbit
    Sample Types: Complex Sample Type
  8. Blueberry Consumption and Changes in Obesity and Diabetes Mellitus Outcomes: A Systematic Review
    Authors: MS de Oliveir, FM Pellenz, BM de Souza, D Crispim
    Metabolites, 2022-12-22;13(1):.
    Species: Mouse
    Sample Types: Serum
  9. Paraoxonase-2 contributes to promoting lipid metabolism and mitochondrial function via autophagy activation
    Authors: GC Shin, HM Lee, N Kim, SK Yoo, HS Park, LS Choi, KP Kim, AR Lee, SU Seo, KH Kim
    Scientific Reports, 2022-12-12;12(1):21483.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Acute effects of exposure to fine particulate matter and ozone on lung function, inflammation and oxidative stress in healthy adults
    Authors: R Xu, Z Li, X Zhu, C Guo, Q Su, J Peng, Z Wang, Y Qian, X Li, Q Xu, Y Wei
    Ecotoxicology and environmental safety, 2022-08-26;243(0):114013.
    Species: Human
    Sample Types: Plasma
  11. Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage
    Authors: B Wang, X Zhang, J Zhong, S Wang, C Zhang, M Li, Q Hu, S Wang, L Chen, W Chen, H Ge, H Feng
    Oxidative Medicine and Cellular Longevity, 2022-08-04;2022(0):6160701.
    Species: Mouse
    Sample Types: Tissue Lysate
  12. Comparison of the Effects of Three Dual-Nucleos(t)ide Reverse Transcriptase Inhibitor Backbones on Placenta Mitochondria Toxicity and Oxidative Stress Using a Mouse Pregnancy Model
    Authors: K Balogun, L Serghides
    Pharmaceutics, 2022-05-15;14(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Clostridioides difficile Toxin B Activates Group 3 Innate Lymphocytes
    Authors: RL Pope, A Chitrakar, P Sah, T Shadid, JD Ballard, LA Zenewicz
    Infection and Immunity, 2022-04-04;0(0):e0007322.
    Species: Rat
    Sample Types: Serum
  14. Minocycline relieves myocardial ischemia-reperfusion injury in rats by inhibiting inflammation, oxidative stress and apoptosis
    Authors: LQ Chen, WS Wang, SQ Li, JH Liu
    European review for medical and pharmacological sciences, 2022-04-01;26(8):3001-3009.
    Species: Rat
    Sample Types: Serum
  15. Fxyd5 activates the NF?kappaB pathway and is involved in chondrocytes inflammation and extracellular matrix degradation
    Authors: L Song, X Li, Q Sun, Y Zhao
    Molecular Medicine Reports, 2022-02-22;25(4):.
    Species: Mouse
    Sample Types: Cell Lysates
  16. Co-Administration of Iron and Bioavailable Curcumin Reduces Levels of Systemic Markers of Inflammation and Oxidative Stress in a Placebo-Controlled Randomised Study
    Authors: H Tiekou Lor, G Begum, L Temouri, D Renshaw, MG Zariwala
    Nutrients, 2022-02-08;14(3):.
    Species: Human
    Sample Types: Serum
  17. Clostridioides difficile Toxin B Activates Group 3 Innate Lymphocytes
    Authors: RL Pope, A Chitrakar, P Sah, T Shadid, JD Ballard, LA Zenewicz
    Infection and Immunity, 2022;0(0):e0007322.
    Species: Rat
    Sample Types: Serum
  18. Ellagic Acid Modulates the Amyloid Precursor Protein Gene via Superoxide Dismutase Regulation in the Entorhinal Cortex in an Experimental Alzheimer's Model
    Authors: WS Ramadan, S Alkarim
    Cells, 2021-12-13;10(12):.
    Species: Rat
    Sample Types: Serum
  19. Mangiferin exert protective effects on joints of adjuvant-induced arthritis rats by regulating the MAPKs/NF-kappaB pathway of fibroblast-like synoviocytes
    Authors: R Wang, J Liu, Z Wang, X Wu, H Guo, X Jiao, H Zhang, C Qi, X Li
    International immunopharmacology, 2021-11-24;0(0):108352.
    Species: Rat
    Sample Types: Plasma
  20. Modulation of COX-2 and NADPH oxidase-4 by alpha-lipoic acid ameliorates busulfan-induced pulmonary injury in rats
    Authors: MG Elhadidy, A Elmasry, HRH Elsayed, M El-Nablawa, S Hamed, MM Elalfy, MR Rabei
    Heliyon, 2021-10-13;7(10):e08171.
    Species: Rat
    Sample Types: Tissue Homogenates
  21. Acute Administration of Bioavailable Curcumin Alongside Ferrous Sulphate Supplements Does Not Impair Iron Absorption in Healthy Adults in a Randomised Trial
    Authors: HT Lorinczova, G Begum, D Renshaw, MG Zariwala
    Nutrients, 2021-07-03;13(7):.
    Species: Human
    Sample Types: Serum
  22. Excessive Iron Induces Oxidative Stress Promoting Cellular Perturbations and Insulin Secretory Dysfunction in MIN6 Beta Cells
    Authors: V Blesia, VB Patel, H Al-Obaidi, D Renshaw, MG Zariwala
    Cells, 2021-05-09;10(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Active food ingredients production from cold pressed processing residues of Camellia oleifera and Camellia sinensis seeds for regulation of blood pressure and vascular function
    Authors: SS Chiang, LS Chen, CY Chu
    Chemosphere, 2020-12-11;267(0):129267.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Effects of the brominated flame retardant, TBCO, on development of zebrafish (Danio rerio) embryos
    Authors: D Van Essen, C Devoy, J Miller, PD Jones, S Wiseman
    Chemosphere, 2020-12-04;266(0):129195.
    Species: Zebrafish
    Sample Types: Embryo, Embryo Homogenate
  25. The impact of maternal obesity and breast milk inflammation on developmental programming of infant growth
    Authors: S Enstad, S Cheema, R Thomas, RN Fichorova, CR Martin, P O'Tierney-, CL Wagner, S Sen
    Eur J Clin Nutr, 2020-08-19;0(0):.
    Species: Human
    Sample Types: Breast Milk, Tissue Homogenates
  26. Nitric oxide and oxidative stress pathways do not contribute to sex differences in renal injury and function in Dahl SS/Jr rats
    Authors: HR Turbeville, AC Johnson, MR Garrett, EL Dent, JM Sasser
    Physiol Rep, 2020-07-01;8(13):e14440.
    Species: Rat
    Sample Types: Urine
  27. Unripe Rubus coreanus Miquel Extract Containing Ellagic Acid Regulates AMPK, SREBP-2, HMGCR, and INSIG-1 Signaling and Cholesterol Metabolism In Vitro and In Vivo
    Authors: KH Lee, ES Jeong, G Jang, JR Na, S Park, WS Kang, E Kim, H Choi, JS Kim, S Kim
    Nutrients, 2020-02-26;12(3):.
    Species: Rat
    Sample Types: Serum
  28. The role of fish oil in attenuating cardiac oxidative stress, inflammation and fibrosis in rat model of thyrotoxicosis
    Authors: F Mayyas, A Alsaheb, KH Alzoubi
    Heliyon, 2019-12-04;5(12):e02976.
    Species: Rat
    Sample Types: Serum
  29. Vinyl chloride-induced interaction of nonalcoholic and toxicant-associated steatohepatitis: Protection by the ALDH2 activator Alda-1
    Authors: L Chen, AL Lang, GD Poff, WX Ding, JI Beier
    Redox Biol, 2019-04-19;24(0):101205.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Dendropanax morbifera Ameliorates Thioacetamide-Induced Hepatic Fibrosis via TGF-?1/Smads Pathways
    Authors: HY Yang, KS Kim, YH Lee, JH Park, JH Kim, SY Lee, YM Kim, IS Kim, S Kacew, BM Lee, JH Kwak, K Yoon, HS Kim
    Int. J. Biol. Sci., 2019-02-13;15(4):800-811.
    Species: Rat
    Sample Types: Tissue Homogenates
  31. Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
    Authors: XJ Chen, SM Ren, JZ Dong, CG Qiu, YW Chen, HL Tao
    Drug Des Devel Ther, 2019-02-13;13(0):647-655.
    Species: Rat
    Sample Types: Tissue Homogenates
  32. STC-1 ameliorates renal injury in diabetic nephropathy by inhibiting the expression of BNIP3 through the AMPK/SIRT3 pathway
    Authors: Z Liu, H Liu, L Xiao, G Liu, L Sun, L He
    Lab. Invest., 2019-01-25;0(0):.
    Species: Human
    Sample Types: Serum
  33. Bone morphogenetic protein 6 (BMP-6) modulates lung function, pulmonary iron levels and cigarette smoke-induced inflammation
    Authors: FM Verhamme, EG De Smet, W Van Hooste, J Delanghe, SE Verleden, GF Joos, GG Brusselle, KR Bracke
    Mucosal Immunol, 2018-12-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. The Effect of Whole-Body Cryotherapy at Different Temperatures on Proinflammatory Cytokines, Oxidative Stress Parameters, and Disease Activity in Patients with Ankylosing Spondylitis
    Authors: A Straburzy?, MP Kasprzak, MW Romanowski, A Kwa?niewsk, W Romanowski, M Iskra, R Rutkowski
    Oxid Med Cell Longev, 2018-10-03;2018(0):2157496.
    Species: Human
    Sample Types: Serum
  35. Efavirenz exposure, alone and in combination with known drugs of abuse, engenders addictive-like bio-behavioural changes in rats
    Authors: M Möller, J Fourie, BH Harvey
    Sci Rep, 2018-08-27;8(1):12837.
    Species: Rat
    Sample Types: Tissue Homogenates
  36. Palmitic acid induces neurotoxicity and gliatoxicity in SH-SY5Y human neuroblastoma and T98G human glioblastoma cells
    Authors: YW Ng, YH Say
    PeerJ, 2018-04-26;6(0):e4696.
    Species: Human
    Sample Types: Cell Lysates
  37. Porphyromonas gingivalis accelerates atherosclerosis through oxidation of high-density lipoprotein
    Authors: HJ Kim, GS Cha, HJ Kim, EY Kwon, JY Lee, J Choi, JY Joo
    J Periodontal Implant Sci, 2018-02-27;48(1):60-68.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Cardiac effects of fish oil in a rat model of streptozotocin-induced diabetes
    Authors: F Mayyas, R Jaradat, KH Alzoubi
    Nutr Metab Cardiovasc Dis, 2018-02-25;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  39. Puerarin protects against heart failure induced by pressure overload through mitigation of ferroptosis
    Authors: B Liu, C Zhao, H Li, X Chen, Y Ding, S Xu
    Biochem. Biophys. Res. Commun., 2018-02-08;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
  40. Pretreatment of ferulic acid attenuates inflammation and oxidative stress in a rat model of lipopolysaccharide-induced acute respiratory distress syndrome
    Authors: S Zhang, P Wang, P Zhao, D Wang, Y Zhang, J Wang, L Chen, W Guo, H Gao, Y Jiao
    Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177505.
    Species: Rat
    Sample Types: Tissue Homogenates
  41. Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model
    Authors: XN Wang, CJ Zhang, HL Diao, Y Zhang
    Chin. Med. J., 2017-05-05;130(9):1026-1032.
    Species: Mouse
    Sample Types: Tissue Homogenates
  42. Randomized trial of red cell washing for the prevention of transfusion-associated organ injury in cardiac surgery
    Authors: MJ Wozniak, N Sullo, S Qureshi, W Dott, R Cardigan, M Wiltshire, T Morris, M Nath, N Bittar, SK Bhudia, T Kumar, AH Goodall, GJ Murphy
    Br J Anaesth, 2017-05-01;118(5):689-698.
    Species: Human
    Sample Types: Serum
  43. Protective effect of active perfusion in porcine models of acute myocardial ischemia
    Mol Med Rep, 2016-08-24;14(4):3581-7.
    Species: Porcine
    Sample Types: Serum
  44. Shape-Related Toxicity of Titanium Dioxide Nanofibres
    Authors: M Allegri, MG Bianchi, M Chiu, J Varet, AL Costa, S Ortelli, M Blosi, O Bussolati, CA Poland, E Bergamasch
    PLoS ONE, 2016-03-21;11(3):e0151365.
    Species: Mouse
    Sample Types: Cell Lysates
  45. The Effect of Krill Oil Supplementation on Exercise Performance and Markers of Immune Function.
    Authors: Da Boit M, Mastalurova I, Brazaite G, McGovern N, Thompson K, Gray S
    PLoS ONE, 2015-09-25;10(9):e0139174.
    Species: Human
    Sample Types: Plasma

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TBARS Parameter Assay Kit
By Mary-Kate Hayward on 03/22/2024
Sample Tested: Mouse tumor

Performed using mouse tumor tissue homogenized in RIPA buffer. Protocol easy to follow and performed well

TBARS Parameter Assay Kit
By Anonymous on 07/13/2017
Sample Tested: Urine

Fast and reliable

TBARS Parameter Assay Kit KGE013
TBARS Parameter Assay Kit
By muthannathb thb on 10/09/2016
Sample Tested: 2120Ep human embryonal carcinoma cell line