Home / TUNEL Assays / TdT In Situ Apoptosis Detection Kit - Core Reagents

TdT In Situ Apoptosis Detection Kit - Core Reagents

Catalog #: 4810-30-CK
13 Citations Datasheet / COA / SDS
In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-CK
Product Details
Citations (13)
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Summary Product Datasheets

TdT In Situ Apoptosis Detection Kit - Core Reagents Summary

Provides a streptavidin-HRP conjugate without an HRP-substrate or a counterstain.
 

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Includes exclusive Cytonin permeabilization reagent.
• Includes TACS-Nuclease control reagents.
• Light microscopy or Fluorescence microscopy options.

Why Use the TdT In Situ Apoptosis Detection Kit - Core Reagents?

TACS 2 TdT Core kit utilize unique cation optimization system to enhance labeling within particular tissues. The kit also features a proprietary labeling buffer that contains no toxic components (e.g. sodium cacodylate). A highly purified form of the TdT enzyme is included for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is detected using streptavidin-horseradish peroxidase, and the researcher's choice of colorimetric substrates or a fluorescent conjugate of streptavidin may be used for visualization by epifluorescence microscopy.

Product Specifications

  • In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Kit Contents

• Streptavidin-HRP
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

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Citations for TdT In Situ Apoptosis Detection Kit - Core Reagents

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Low-Molecular-Weight beta-1,3-1,6-Glucan Derived from Aureobasidium pullulans Exhibits Anticancer Activity by Inducing Apoptosis in Colorectal Cancer Cells
    Authors: JH Kim, J Seo, H No, T Kuge, T Mori, H Kimoto, JK Kim
    Biomedicines, 2023-02-11;11(2):.  2023-02-11
  2. SARS-CoV-2 recombinant spike protein induces cell apoptosis in rat taste buds
    Authors: T Yamamoto, Y Koyama, T Ujita, E Sawada, N Kishimoto, K Seo
    Journal of dental sciences, 2022-08-26;0(0):.  2022-08-26
  3. Combined Caloric Restriction and Exercise Provides Greater Metabolic and Neurocognitive Benefits than either as a Monotherapy in Obesity With or Without Estrogen Deprivation
    Authors: W Pratchayas, B Arunsak, K Suparan, S Sriwichaii, T Chunchai, N Chattipako, SC Chattipako
    The Journal of nutritional biochemistry, 2022-08-14;0(0):109125.  2022-08-14
  4. Disulfiram inhibits liver fibrosis in rats by suppressing hepatic stellate cell activation and viability
    Authors: XM Yang, Z Wu, X Wang, Y Zhou, L Zhu, D Li, HZ Nie, YH Wang, J Li, X Ma
    Oncogene, 2022-07-22;23(1):54.  2022-07-22
  5. Improved bladder contractility after transplantation of human mesenchymal stem cells overexpressing hepatocyte growth factor into underactive bladder from bladder outlet obstruction models of rats
    Authors: JH Kim, HJ Yang, SS Choi, SU Kim, HJ Lee, YS Song
    PLoS ONE, 2021-12-22;16(12):e0261402.  2021-12-22
  6. The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation
    Authors: Y Ikeda, A Tagami, M Maekawa, A Nagai
    Scientific Reports, 2021-02-24;11(1):4486.  2021-02-24
  7. Decabromodiphenyl ether (BDE-209) exposure to lactating mice perturbs steroidogenesis and spermatogenesis in adult male offspring
    Authors: D Sarkar, SK Singh
    Ecotoxicology and environmental safety, 2020-12-29;209(0):111783.  2020-12-29
  8. Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma
    Authors: X Wang, EM Langer, CJ Daniel, M Janghorban, V Wu, XJ Wang, RC Sears
    Oncogenesis, 2020-09-07;9(9):79.  2020-09-07
  9. Smad7 induces plasticity in tumor-infiltrating Th17 cells and enables TNF-alpha-mediated killing of colorectal cancer cells.
    Authors: Rizzo A, De Mare V, Rocchi C, Stolfi C, Colantoni A, Neurath M, MacDonald T, Pallone F, Monteleone G, Fantini M
    Carcinogenesis, 2014-01-30;35(7):1536-46.  2014-01-30
  10. Differential effects of 2- and 3-series E-prostaglandins on in vitro expansion of Lgr5+ colonic stem cells.
    Authors: Fan Y, Davidson L, Callaway E, Goldsby J, Chapkin R
    Carcinogenesis, 2013-12-11;35(3):606-12.  2013-12-11
  11. Autophagy in pancreatic acinar cells in caerulein-treated mice: immunolocalization of related proteins and their potential as markers of pancreatitis.
    Authors: Zhang L, Zhang J, Shea K, Xu L, Tobin G, Knapton A, Sharron S, Rouse R
    Toxicol Pathol, 2013-05-02;42(2):435-57.  2013-05-02
  12. Under-expression of the opioid growth factor receptor promotes progression of human ovarian cancer.
    Authors: Donahue R, McLaughlin P, Zagon I
    Exp Biol Med (Maywood), 2012-02-10;237(2):167-77.  2012-02-10
  13. Age-dependent demise of GNAS-mutated skeletal stem cells and "normalization" of fibrous dysplasia of bone.
    Authors: Kuznetsov SA, Cherman N, Riminucci M, Collins MT, Robey PG, Bianco P
    J. Bone Miner. Res., 2008-11-01;23(11):1731-40.  2008-11-01

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