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Tyrosine Hydroxylase Antibody

Catalog #: MAB1423
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB1423 has been discontinued.
View all Tyrosine Hydroxylase products.
Detection of Tyrosine Hydroxylase by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Tyrosine Hydroxylase Antibody Summary

Specificity
Detects rodent, monkey, bovine, sheep, rabbit, guinea pig, and human tyrosine hydroxylase. It recognizes an epitope present in the N‑terminal region (approximately amino acids 9-16) of tyrosine hydroxylase.
Source
Monoclonal Mouse IgG1 Clone # TH-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat tyrosine hydroxylase
Formulation

Supplied as a 0.2 μm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of rat brain
Immunoprecipitation
Arita, D. et al. (2002) J. Cell Biochem. 87:58.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Tyrosine Hydroxylase antibody by Western Blot. View Larger

Detection of Tyrosine Hydroxylase by Western Blot. Western blot shows lysates of rat brain tissue under reducing and non-reducing conditions. PVDF membrane was probed with 2 µg/mL Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB1423) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Tyrosine Hydroxylase was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Tyrosine Hydroxylase

Tyrosine hydroxylase is a tetrameric enzyme that catalyzes the hydroxylation of L-tyrosine to L-3,4-dihydroxyphenyalanine (L-dopa) in brain and andrenal medulla. This is the rate-limiting step in the biosynthesis of catecholamines, such as dopamine, norepinephrine, and epinephrine that serve as neurotransmitters and hormones (1).

References
  1. Nagatsu, T. et al. (1964) J. Biol. Chem. 239:2910
  2. Arita, D. et al. (2002) J. Cell Biochem. 87:58.
Entrez Gene IDs
7054 (Human); 21823 (Mouse); 25085 (Rat)
Alternate Names
DYT14; DYT5b; EC 1.14.16; EC 1.14.16.2; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase

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Citation for Tyrosine Hydroxylase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A combined epigenetic and non-genetic approach for reprogramming human somatic cells.
    Authors: Han J, Sachdev PS, Sidhu KS
    PLoS ONE, 2010-08-19;5(8):e12297.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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