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Viral EBOV GP Antibody

Catalog #: MAB9016
2 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9016-100
MAB9016-SP
Detection of EBOV GP by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral EBOV GP Antibody Summary

Species Reactivity
Viral
Specificity
Detects EBOV GP in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2A Clone # 993408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant Viral EBOV GP
Ile33-Arg501
Accession # Q05320
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of EBOV GP antibody by Western Blot. View Larger

Detection of EBOV GP by Western Blot. Western blot shows Recombinant EBOV GP. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral EBOV GP Monoclonal Antibody (Catalog # MAB9016) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EBOV GP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBOV GP

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

References
  1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
  2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
  3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
  4. Volchkov, V.E. et al. (1998) Virology 245:110.
  5. Sanchez, A. et al. (1998) J. Virol. 72:6442.
  6. Dolnik, O. et al. (2004) EMBO J. 23:2175.
  7. Takada, A. et al. (2004) J. Virol. 78:2943.
  8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
  9. Simmons, G. et al. (2003) Virology 305:115.
  10. Schornberg, K. et al. (2006) J. Virol. 80:4174.
  11. Chandran, K. et al. (2005) Science 308:1643.
  12. Cote, M. et al. (2011) Nature 477:344.
Long Name
Ebola Virus Glycoprotein
Entrez Gene IDs
911829 (Viral)
Alternate Names
bovGP; EBOV GP; second secreted glycoprotein; small secreted glycoprotein; Spike glycoprotein

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Citations for Viral EBOV GP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein
    Authors: E Paparisto, NR Hunt, DS Labach, MD Coleman, EJ Di Gravio, MJ Dodge, NJ Friesen, M Côté, A Müller, T Hoenen, SD Barr
    Cells, 2021-09-13;10(9):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. IgY antibodies against Ebola virus possess post-exposure protection in a murine pseudovirus challenge model and excellent thermostability
    Authors: Y Zhang, Y Wei, Y Li, X Wang, Y Liu, D Tian, X Jia, R Gong, W Liu, L Yang
    PloS Neglected Tropical Diseases, 2021-03-12;15(3):e0008403.
    Species: Hamster, Human, Primate
    Sample Types: Cell Lysates
    Applications: Western Blot

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