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Viral HCMV IL-10 Antibody

Catalog #: AF117
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF117
AF117-SP
IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral HCMV IL-10 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral HCMV IL-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑10, recombinant canine IL‑10, recombinant feline IL‑10, recombinant cotton rat IL‑10, recombinant rat IL‑10, recombinant porcine IL‑10, recombinant human IL‑10, and recombinant Epstein-Barr virus encoded viral IL‑10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant viral HCMV IL‑10
Ala26-Lys176
Accession # AAF63437
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Viral HCMV IL‑10 (Catalog # 117-VL)
Neutralization
Measured by its ability to neutralize IL‑10-mediated inhibition of LPS-induced IL-1 beta secretion in human peripheral blood lymphocytes (PBL). The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 2 ng/mL Recombinant Viral HCMV IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. View Larger

IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. Recombinant Viral HCMV IL-10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND50 is typically 0.15-0.9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

References
  1. Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
  2. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
  3. Spencer, J.V. et al. (2002) J. Virol. 76:1285.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Viral HCMV IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Cytomegalovirus proteins, maternal pregnancy cytokines, and their impact on neonatal immune cytokine profiles and acute lymphoblastic leukemogenesis in children
    Authors: Joseph L. Wiemels, Rong Wang, Mi Zhou, Helen Hansen, Rachel Gallant, Junghyun Jung et al.
    Haematologica
  2. Challenging the Conventional Interpretation of HCMV Seronegativity
    Authors: S Waters, S Lee, A Irish, P Price
    Microorganisms, 2021-11-18;9(11):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  3. Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
    Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
    Sci Rep, 2017-01-05;7(0):39710.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  4. cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
    Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
    PLoS ONE, 2014-02-10;9(2):e88708.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
    Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
    BMC Biotechnol, 2013-10-05;13(0):82.
    Species: Virus
    Sample Types: Cell Fraction
    Applications: ELISA Development, Western Blot
  6. Gliomas Promote Immunosuppression through Induction of B7-H1 Expression in Tumor-Associated Macrophages
    Authors: Orin Bloch, Courtney A. Crane, Rajwant Kaur, Michael Safaee, Martin J. Rutkowski, Andrew T. Parsa
    Clinical Cancer Research
  7. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity
    Authors: Noelle D. Brodeur, Juliet V. Spencer
    Virus Research
  8. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
    Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
    J. Virol., 2008-01-23;82(7):3736-50.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Western Blot
  9. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004-08-01;78(16):8720-31.
    Species: Virus
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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