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Viral HCMV IL-10 Biotinylated Antibody

Catalog #: BAF117
9 Citations Datasheet / COA / SDS
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BAF117
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Citations (9)
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Viral HCMV IL-10 Biotinylated Antibody Summary

Species Reactivity
Viral
Specificity
Detects Viral HCMV IL-10 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant Viral HCMV IL‑10 (R&D Systems, Catalog # 117-VL)
Ala26-Lys176
Accession # AAF63437
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Viral HCMV IL‑10 (Catalog # 117-VL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

References
  1. Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
  2. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
  3. Spencer, J.V. et al. (2002) J. Virol. 76:1285.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Viral HCMV IL-10 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Human Cytomegalovirus pUL11, a CD45 Ligand, Disrupts CD4 T Cell Control of Viral Spread in Epithelial Cells
    Authors: SA Osanyinlus, J Zischke, R Jacobs, EM Weissinger, TF Schulz, PC Kay-Fedoro
    MBio, 2022-11-29;0(0):e0294622.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Cytomegalovirus proteins, maternal pregnancy cytokines, and their impact on neonatal immune cytokine profiles and acute lymphoblastic leukemogenesis in children
    Authors: Joseph L. Wiemels, Rong Wang, Mi Zhou, Helen Hansen, Rachel Gallant, Junghyun Jung et al.
    Haematologica
  3. Challenging the Conventional Interpretation of HCMV Seronegativity
    Authors: S Waters, S Lee, A Irish, P Price
    Microorganisms, 2021-11-18;9(11):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  4. Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
    Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
    Sci Rep, 2017-01-05;7(0):39710.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  5. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
    Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
    BMC Biotechnol, 2013-10-05;13(0):82.
    Species: Virus
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Gliomas Promote Immunosuppression through Induction of B7-H1 Expression in Tumor-Associated Macrophages
    Authors: Orin Bloch, Courtney A. Crane, Rajwant Kaur, Michael Safaee, Martin J. Rutkowski, Andrew T. Parsa
    Clinical Cancer Research
  7. Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
    Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
    Clin. Cancer Res., 2011-04-13;17(14):4642-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004-08-01;78(16):8720-31.
    Species: Virus
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: ELISA Development, Western Blot

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