Viral HHV8-ORF74 Antibody

Catalog #: MAB5264 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5264
MAB5264-SP

All HHV8-ORF74 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral HHV8-ORF74 Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral HHV8-ORF74.

Source

Monoclonal Mouse IgG_2B Clone # 462510

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with viral HHV8-ORF74
Met1-Thr342
Accession # AAB51506

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

NS0 mouse myeloma cell line transfected with viral HHV8-ORF74

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HHV8-ORF74

HHV8‑ORF74 is a 342 amino acid viral 7-transmembrane receptor encoded by the Kaposi's sarcoma-associated herpesvirus 8. The G‑protein-coupled receptor is a constitutively active homologue of CXCR2, the CXCL8 receptor (IL-8 RB). It contributes to Kaposi's sarcoma by triggering expression of paracrine factors such as VEGF-A, inflammatory cytokines, adhesion molecules and chemokines, and enhancing cell growth.

Long Name

Human Herpesvirus 8 ORF74

Entrez Gene IDs

4961460 (Viral)

Alternate Names

HHV8ORF74; HHV8-ORF74; KSHV-vGPCR