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Viral MCV-type II Chemokine-like Protein Antibody

Catalog #: AF697 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF697
AF697-SP
MCV‑type II Chemokine‑like Protein Inhibition of CCL1/I‑309-dependent Chemotaxis and Neutralization by Viral MCV‑type II Chemokine‑like Protein Antibody.
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Viral MCV-type II Chemokine-like Protein Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral MCV-type II Chemokine-like Protein in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant viral MCV-type II Chemokine-like Protein
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Viral MCV-type II Chemokine-like Protein (Catalog # 697-M3)
Neutralization
Measured by its ability to neutralize MCV‑type II Chemokine‑like Protein inhibition of CCL1/I‑309-dependent chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.2 µg/mL Recombinant Viral MCV‑type II Chemokine‑like Protein and 20 #ng/mL Recombinant Human CCL1/I‑309.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization MCV‑type II Chemokine‑like Protein Inhibition of CCL1/I‑309-dependent Chemotaxis and Neutralization by Viral MCV‑type II Chemokine‑like Protein Antibody. View Larger

MCV‑type II Chemokine‑like Protein Inhibition of CCL1/I‑309-dependent Chemotaxis and Neutralization by Viral MCV‑type II Chemokine‑like Protein Antibody. Recombinant Viral MCV-type II Chemokine-like Protein (Catalog # 697-M3) inhibits Recombinant Human CCL1/I-309 (Catalog # 272-I) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human CCL1/I-309 (20 ng/mL) activity elicited by Recombinant Viral MCV-type II Chemokine-like Protein (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral MCV-type II Chemokine-like Protein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF697). The ND50 is typically 5-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCV-type II Chemokine-like Protein

Molluscum contagiosum virus (MCV) is a human cutaneous poxvirus which causes benign proliferative lesions of the skin in normal and immunocompromised individuals. Based on endonuclease cleavage patterns of viral DNA, MCV isolates can be classified into two different types, MCV type I (MCVI) and MCV type II (MCVII). Like other DNA viruses such as poxviruses and herpesviruses, MCV has been shown to encode homologs of cellular immunomodulatory proteins. These viral proteins enable the virus to evade the host immune system and enhance the ability of the virus to propogate and survive.

MC148R2 is a CC chemokine-like protein encoded by a MCV type II gene. MC148R2 cDNA encodes a putative 104 amino acid (aa) protein containing a 24 aa signal peptide that is cleaved to produce an 80 aa mature protein. The mature protein shares 89% homology to the MCV type I chemokine-like protein, MC148R1. Compared to MIP-beta, MC148R2 retains the 4 conserved cysteine residues but has a 5 aa residue deletion at the amino-terminus region which is important for receptor activation. The MCV type I and type 2 proteins do not exhibit chemotactic properties, however these proteins have been shown in vitro to inhibit the monocyte chemotaxis induced by human MIP-1 alpha as well as the binding of radiolabeled I-309 to hCCR8 transfected cells.

References
  1. Krathwohl, M. et al. (1997) Proc. Natl. Acad. Sci. USA 94:9875.
  2. Senkevich, T. et al. (1996) Science 273:813.
  3. Dairaghi, D.J. et al. (1999) J. Biol. Chem. 274:21569.
Long Name
Molluscum Contagiosum Virus Type II
Alternate Names
MCVtype II; MCV-type II

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