Viral Tick-borne Encephalitis Virus NS1 Antibody

Catalog #: MAB101061 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB101061-100
MAB101061-SP

Detection of Tick-borne Encephalitis Virus NS1 by Western Blot.

2 Images

All Tick-borne Encephalitis Virus NS1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral Tick-borne Encephalitis Virus NS1 Antibody Summary

Species Reactivity

Viral

Specificity

Detects Viral Tick-borne Encephalitis Virus NS1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1004120

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived viral Tick-borne Encephalitis Virus NS1
Amino Acids 777-1128

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB10106).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Tick-borne Encephalitis Virus NS1 by Western Blot. Western blot shows recombinant tick-borne encephalitis virus (TBEV). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB101061) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tick-borne Encephalitis Virus at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA

View Larger

Viral Tick-borne Encephalitis Virus NS1 ELISA Standard Curve. Recombinant Viral Tick-borne Encephalitis Virus NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB101061) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB10106) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tick-borne Encephalitis Virus NS1

Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus, is the virus associated with tick-borne encephalitis. Three TBEV sub-types have been described: European or Western tick-borne encephalitis virus, Siberian tick-borne encephalitis virus, and Far eastern Tick-borne encephalitis virus (formerly known as Russian Spring Summer encephalitis virus, RSSEV). TEBV NS1 protein is one of the most conserved. Homology level of the NS1 protein is about 90% between TBEV strains. Tick-borne encephalitis virus causes 13,000 cases of human meningitis and encephalitis annually.

Entrez Gene IDs

1489719 (Viral)

Alternate Names

TBEV NS1; TBEV-NS1; Tick-borne Encephalitis Virus NS1