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Viral wnvNS3 Protease Antibody

Catalog #: AF2907
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2907
AF2907-SP
Detection of wnvNS3 Protease by Western Blot.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral wnvNS3 Protease Antibody Summary

Species Reactivity
Viral
Specificity
Detects West Nile Virus NS3 Protease in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L
Gly1506-Leu1689
Accession # ABD85079
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907‑SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of wnvNS3 Protease antibody by Western Blot. View Larger

Detection of wnvNS3 Protease by Western Blot. Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Viral wnvNS3 Protease Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

References
  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name
West Nile Virus NS3 Protease
Entrez Gene IDs
912267 (Viral)
Alternate Names
wnvNS3 Protease

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Citations for Viral wnvNS3 Protease Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. West Nile Virus Infection Induces Depletion of IFNAR1 Protein Levels
    Authors: Jared D. Evans, Rachel A. Crown, Ji A. Sohn, Christoph Seeger
    Viral Immunology
  2. The A946T variant of the RNA sensor IFIH1 mediates an interferon program that limits viral infection but increases the risk for autoimmunity
    Authors: Jacquelyn A. Gorman, Christian Hundhausen, John S. Errett, Amy E. Stone, Eric J. Allenspach, Yan Ge et al.
    Nature Immunology
  3. TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation
    Authors: AI Chiramel, NR Meyerson, KL McNally, RM Broeckel, VR Montoya, O Méndez-Sol, SJ Robertson, GL Sturdevant, KJ Lubick, V Nair, BH Youseff, RM Ireland, CM Bosio, K Kim, J Luban, VM Hirsch, RT Taylor, F Bouamr, SL Sawyer, SM Best
    Cell Rep, 2019-06-11;27(11):3269-3283.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  4. Interleukin-1? Signaling in Dendritic Cells Induces Antiviral Interferon Responses
    Authors: LD Aarreberg, C Wilkins, HJ Ramos, R Green, MA Davis, K Chow, M Gale
    MBio, 2018-03-20;9(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Arsenite-induced stress granule formation is inhibited by elevated levels of reduced glutathione in West Nile virus-infected cells
    Authors: M Basu, SC Courtney, MA Brinton
    PLoS Pathog, 2017-02-27;13(2):e1006240.
    Species: Hamster
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling.
    Authors: Lazear H, Lancaster A, Wilkins C, Suthar M, Huang A, Vick S, Clepper L, Thackray L, Brassil M, Virgin H, Nikolich-Zugich J, Moses A, Gale M, Fruh K, Diamond M
    PLoS Pathog, 2013-01-03;9(1):e1003118.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice.
    Oncogene, 2012-05-23;86(15):7953-63.
    Species: Virus
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.
    Authors: Wang P, Arjona A, Zhang Y
    Nat. Immunol., 2010-09-05;11(10):912-9.
    Species: Virus
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Zika Virus Infection Induces DNA Damage Response in Human Neural Progenitors That Enhances Viral Replication
    Authors: Christy Hammack, Sarah C. Ogden, Joseph C. Madden, Angelica Medina, Chongchong Xu, Ernest Phillips et al.
    Journal of Virology

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