Viral Zika Virus (H/PF/2013) NS1 Antibody

Catalog #: MAB99202 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB99202-100
MAB99202-SP

Detection of Viral Zika Virus (H/PF/2013) NS1 by Western Blot.

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All Zika Virus (H/PF/2013) NS1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral Zika Virus (H/PF/2013) NS1 Antibody Summary

Species Reactivity

Viral

Specificity

Detects Viral Zika Virus (H/PF/2013) in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 990910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived recombinant Viral Zika Virus (H/PF/2013) NS1
Val796 & Ser799-Ser1148
Accession # AHZ13508

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Viral Zika Virus (H/PF/2013) NS1 by Western Blot. Western blot shows lysates of Recombinant Viral Zika Virus (H/PF/2013) NS1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB99202) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Zika Virus (H/PF/2013) NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Zika Virus (H/PF/2013) NS1

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host. ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

References