West Nile Virus NS3 Protease Biotinylated Antibody Summary
Gly1506-Leu1689
Accession # ABD85079
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: wnvNS3 Protease
Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.
Product Datasheets
Citations for West Nile Virus NS3 Protease Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection
Authors: Amy E. L. Stone, Richard Green, Courtney Wilkins, Emily A. Hemann, Michael Gale
Nature Communications
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Early cellular innate immune responses drive Zika viral persistence and tissue tropism in pigtail macaques
Authors: MA O'Connor, J Tisoncik-G, TB Lewis, CJ Miller, D Bratt, CR Moats, PT Edlefsen, J Smedley, NR Klatt, M Gale, DH Fuller
Nat Commun, 2018-08-22;9(1):3371.
Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry -
Infectious clones of novel lineage 1 and lineage 2 West Nile virus strains WNV-TX02 and WNV-Madagascar.
J. Virol., 2012-05-09;86(14):7704-9.
Species: Virus
Sample Types: Cell Lysates
Applications: Western Blot -
Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis
Authors: Dokyoon Kim, Changlin Fu, Xuefeng B Ling, Zhongkai Hu, Guozhong Tao, Yingzhen Zhao et al.
Journal of Proteomics & Bioinformatics
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