Zebrafish BMP-2 Antibody

Catalog #: MAB111 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB111
MAB111-SP

Alkaline Phosphatase Production Induced by BMP‑2a and Neutralization by Zebrafish BMP‑2 Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Zebrafish BMP-2 Antibody Summary

Species Reactivity

Zebrafish

Specificity

Detects zebrafish BMP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human BMP-2, -3, -3B,
-4, -5, -6, -7, -8, or recombinant zebrafish BMP-4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 208301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant zebrafish BMP‑2
Gln272-Arg386
Accession # AAI63048

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Zebrafish BMP‑2a (Catalog # 111-BM)

Neutralization

Measured by its ability to neutralize BMP‑2a-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND₅₀) is typically 5-25 µg/mL in the presence of 1 µg/mL Recombinant Zebrafish BMP‑2a and 2 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Alkaline Phosphatase Production Induced by BMP‑2a and Neutralization by Zebrafish BMP‑2 Antibody. Recombinant Zebrafish BMP-2a (Catalog # 111-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Zebrafish BMP-2a (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Zebrafish BMP-2 Monoclonal Antibody (Catalog # MAB111). The ND₅₀ is typically 5-25 µg/mL in the presence of heparin (2 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2

BMP-2 is one of at least 20 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF‑ beta ) superfamily (1, 2). BMPs were originally identified as protein regulators of cartilage and bone formation. However, they are also involved in embryogenesis and morphogenesis of various tissues and organs. BMPs regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse, and rat BMP-2 share 100% amino acid (aa) sequence identity, while mature human BMP-2 and zebrafish BMP-2 share 85% aa identity. Zebrafish have another homolog of BMP-2 (also called BMP-2a) called BMP-2b, which is 88% identical to BMP-2 in the mature region and corresponds to the swirl mutant. The combined expression pattern of zfBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates (3). Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 115 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero‑ogliomeric complexes of type I and type II serine/threonine kinase receptors (4). In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both type I BMP receptor’s can bind BMP-2 with high affinity in the absence of BMP receptor type II.

References