Immunohistochemistry (IHC) Protocols

Troubleshooting Guide: Immunohistochemistry

Technically, IHC and ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be identified and optimized for each individual IHC/ICC study. Optimization of IHC/ICC may also require troubleshooting a variety of factors. The tables below highlight common IHC/ICC issues and provide appropriate experimental actions. For further assistance, please contact our technical service department.

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections

Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections