Home / IkB-alpha / Human Phospho-IkB-alpha (S32/S36) Antibody

Human Phospho-IkB-alpha (S32/S36) Antibody

Catalog #: AF4809
4 Citations Datasheet / COA / SDS

Discontinued Product

AF4809 has been discontinued.
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Detection of Human Phospho-IkB‑ alpha (S32/S36) by Western Blot.
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Citations (4)
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Human Phospho-IkB-alpha (S32/S36) Antibody Summary

Species Reactivity
Human
Specificity
Detects human IkB-alpha when phosphorylated at S32/36.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human IkB-alpha S32/36 sites
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-IkB-a (S32/S36) antibody by Western Blot. View Larger

Detection of Human Phospho-IkB‑ alpha (S32/S36) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 5 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-IkB-a (S32/S36) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4809) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-IkB-a (S32/S36) was detected at approximately 44 kDa (as indicated). For additional reference, duplicate lysates were probed with 0.1 µg/mL Human IkB-a Monoclonal Antibody (lower panel, Catalog # MAB4299). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-alpha

NF kappa B inhibitor alpha (IkB‑ alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues 32 and 36 marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name
I-kappa-B-alpha
Entrez Gene IDs
4792 (Human); 18035 (Mouse); 25493 (Rat)
Alternate Names
alpha; I-kappa-B-alpha; IkappaBalphaNF-kappa-B inhibitor alpha; IKBAikB-alpha; IkBalpha; IkB-alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

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Citations for Human Phospho-IkB-alpha (S32/S36) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. An ultrasensitive fiveplex activity assay for cellular kinases
    Authors: CM Smolko, KA Janes
    Sci Rep, 2019-12-19;9(1):19409.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Multiplex Assay
  2. Cordycepin Enhances Radiosensitivity in Oral Squamous Carcinoma Cells by Inducing Autophagy and Apoptosis Through Cell Cycle Arrest
    Authors: SY Ho, WS Wu, LC Lin, YH Wu, HW Chiu, YL Yeh, BM Huang, YJ Wang
    Int J Mol Sci, 2019-10-28;20(21):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation
    Authors: K Seno, Y Munakata, M Sano, R Kawahara-M, H Takahashi, A Ohkuchi, H Iwata, T Kuwayama, K Shirasuna
    Int J Mol Sci, 2018-08-08;19(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
    Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
    PLoS ONE, 2014-06-24;9(6):e100787.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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