A reaction mixture containing a diphosphonucleotide sugar donor, an acceptor substrate, and CD39L3 is added to each well of a 96-well microplate.
The reaction is initiated by adding the glycosyltransferase to each well of the microplate.
The Malachite Green Phosphate Detection Reagents are added to the wells. The reactions are terminated and a green color develops in proportion to the amount of inorganic phosphate released by the coupling phosphatase. The absorbance of the color at 620 nm is measured.
