DNA fragmentation represents a characteristic hallmark of apoptosis. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is an established method for detecting DNA fragments.
The TACS® TdT kits contain a highly purified form of the TdT enzyme for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is achieved using Streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB) or TACS Blue Label™.