Luminex assays use a set of fluorescent beads where each bead falls in a unique spot on the fluorescent spectrum. The beads are coated with capture antibody targeted to the analyte of interest.
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| The sample is added to a mixture of color-coded beads, pre-coated with analyte-specific capture antibodies. The antibodies bind to the analytes of interest. |
Biotinylated detection antibodies specific to the analytes of interest are added and form an antibody-antigen sandwich. Phycoerythrin (PE)-conjugated streptavidin is added. It binds to the biotinylated detection antibodies. |
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| A magnet in the Luminex instrument captures and holds the magnetic beads in a monolayer, while two spectrally distinct light-emitting diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal. Each well is imaged with a CCD camera. |
Benefits of Luminex Multiplex Assays
- Small Sample Volume: Conserves sample by measuring multiple analytes in a single well with less than 50 µL of sample.
- Save Time and Money: Save resources when compared to running multiple single-analyte ELISAs.
- Flexibility: Build analyte panels to fit your research needs.
- Low Variability: Minimizes experimental variability with multiple data points derived from a single experiment.
Benefits of R&D Systems Luminex Assays
R&D Systems’ Luminex assays have the largest, most flexible Luminex analytes available. With our menu of off-the-shelf fixed panels, high-performance configurable panels, and completely custom panels, we have options to best fit your needs, ensuring you get the most data from your samples with results you can trust.
- Selection: Flexibility of panels and formats available.
- Experience: High Performance Luminex Assays are correlated to the gold standard Quantikine ELISA.
- Availability: Fast delivery times.
- Superior Quality Control:Assays undergo multiple quality control steps during production ensuring long-term reproducibility and consistency.
- Specific: Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.
- Validation: High performance panels are extensively validated for optimal performance.
- Versatile: Antibody pairs are tested to confirm parallel detection of natural and recombinant proteins.
- Custom Services: Expert custom team available to help meet your unique needs.
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Luminex®, Luminex® 200™, FLEXMAP 3D®, and xMAP INTELLIFLEX® are trademarks of Luminex Corporation.
