Products for Regulatory T Cell Research

R&D Systems has recently introduced several new products for regulatory T (Treg) cell research, including a MagCellect^TM Human CD4⁺CD25⁺ Regulatory T Cell Selection Kit, a Mouse CD4⁺CD25⁺ Regulatory T Cell Selection Kit, along with a Human Regulatory T Cell Multi-Color Flow Cytometry Kit, a Mouse Regulatory T Cell Multi-Color Flow Cytometry Kit, and Rat Regulatory T Cell Multi-Color Flow Cytometry Kit (Catalog # FMC021, FMC022, FMC015B, respectively). The Human and Mouse Treg cell selection kits are designed to isolate CD4⁺CD25⁺ Treg cells from PBMCs or a splenocyte suspension, respectively, using a two-step procedure that combines both negative and positive selection techniques. CD4⁺ cells are initially enriched by negative se­lection, whereby unwanted cells are tagged and magnetically removed. CD25⁺ cells are sub­sequently isolated from the enriched CD4⁺ population by positive selection. Using these kits, the purity of the isolated CD4⁺CD25⁺ population is typically between 60-70%.

For identification of Treg cells by flow cytometry, we also now offer Treg Multi-Color Flow Cytometry Kits for several different species. These kits include fluorochrome-conjugated antibodies against CD4, CD25, and FoxP3 that can be used together for single-step staining of human, mouse, or rat Treg cells. In addition, several new proteins and fluorochrome-conjugated antibodies (Table 1) add to a growing list of reagents that are available for functional studies on Treg cells. For a complete listing of Treg-related products, please visit our website at Regulatory T Cells (Tregs) Molecule Listing.

Table 1. New Proteins & Antibodies for Treg Cell Research

** These antibodies, along with R&D Systems Mouse Anti-Human/Mouse IL-12/IL-35 p35 Monoclonal Antibody
(Catalog # MAB1570), were cited in the most highly ranked article in immunology from the Faculty of 1000 Post-Publication Peer Review published by The Scientist.^{1, 2}

1. Zielinska, E. (2011) The Scientist [Epub Feb. 8].
2. Collison, L.W. et al. (2010) Nat. Immunol. 11:1093.

This symbol denotes references that cite the use of R&D Systems products.

MagCellect is a registered trademark of R&D Systems, Inc. Alexa Fluor is a registered trademark of Life Technologies.