Human CRISP-3 Biotinylated Antibody

Catalog #: BAF2397 Datasheet / COA / SDS

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BAF2397

All CRISP-3 Antibodies

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Human CRISP-3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRISP-3 in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human CRISP-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRISP-3
Asn21-Tyr245 (Ser134Ala)
Accession # CAA63984

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CRISP‑3 (Catalog # 2397-CR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-3

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1‑3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, however, CRISP-3 has not been detected in the male genital tract (8, 9). CRISP-3 is up-regulated in epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta ‑microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes (14, 15). CRISP-3 is released from neutrophil and eosinophil granules following cell stimulation (1, 15). Mature human CRISP-3 shares 48% and 65% amino acid (aa) sequence identity with mouse and equine CRISP-3, respectively. It shares 44% and 72% aa sequence identity with human CRISP-1 and -2, respectively.

References

Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
Udby, L. et al. (2005) J. Androl. 26:333.
Haendler, B. et al. (1993) Endocrinology 133:192.
Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
Bjartell, A. et al. (2006) Prostate 66:591.
Liao, Q. et al. (2003) Histol. Histopathol. 18:245.
Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
Udby, L. et al. (2004) Biochemistry 43:12877.
Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.

Long Name

Cysteine-rich Secretory Protein 3

Entrez Gene IDs

10321 (Human); 11572 (Mouse)

Alternate Names

Aeg2; CRISP3; CRISP-3; CRISP-3MGC126588; CRS3; cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; dJ442L6.3; SGP28; SGP28Aeg2; specific granule protein (28 kDa); Specific granule protein of 28 kDa