Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ENPP-7/Alk-SMase Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Ala22-Ser439
Accession # Q6UWV6

Specificity

Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ENPP-7 is observed, and less than 5% cross-reactivity with recombinant human (rh) ENPP-2 and rhENPP-5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human ENPP‑7/Alk‑SMase Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ENPP‑7/Alk-SMase (Catalog # 4924-EN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ENPP‑7/Alk-SMase (Catalog # 4924-EN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ENPP-7/Alk-SMase

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (alk-SMase) is expressed in the intestines and in human bile (1). It shares 30%‑36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP-7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies also show a decrease in ENPP-7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP-7 in human colon cancer (4, 5).

References

  1. Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
  2. Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
  3. Wu, J. et al. (2006) Biochem J. 394:299.
  4. Hertervig, E. et al. (1996) Cancer. 79:448.
  5. Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Alternate Names

Alk-SMase, ENPP7, NPP7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Gene Symbol

ENPP7

UniProt

Q6UWV6

Additional ENPP-7/Alk-SMase Products

Product Documents for Human ENPP‑7/Alk‑SMase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ENPP‑7/Alk‑SMase Antibody

For research use only

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