Human IGF-I/IGF-1 Biotinylated Antibody Summary
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Applications
Human IGF-I/IGF-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IGF-I/IGF-1 by Simple WesternTM. Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF291). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4 - 7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).
- Philippou, A. et al. (2007) In Vivo 21:45.
- Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
- Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
- Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
- Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
- Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
- Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
- Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
- LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
- Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Product Datasheets
Citations for Human IGF-I/IGF-1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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ATM-dependent IGF-1 induction regulates secretory clusterin expression after DNA damage and in genetic instability
Authors: E M Goetz, B Shankar, Y Zou, J C Morales, X Luo, S Araki et al.
Oncogene
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Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
Adv Healthc Mater, 2018-02-14;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Detection -
Calling Biomarkers in Milk Using a Protein Microarray on Your Smartphone.
Authors: Ludwig S, Tokarski C, Lang S, van Ginkel L, Zhu H, Ozcan A, Nielen M
PLoS ONE, 2015-08-26;10(8):e0134360.
Species: Bovine
Sample Types: Milk
Applications: Array Development -
ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence.
Authors: Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D
PLoS ONE, 2014-06-17;9(6):e99983.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
Clin. Cancer Res., 2007-02-01;13(3):1000-9.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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