Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MST1/STK4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MST1/STK4
Lys285-Asp443
Accession # Q13043

Specificity

Detects human MST1/STK4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human MST2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MST1/STK4 Antibody

Detection of Human MST1/STK4 antibody by Western Blot.

Detection of Human MST1/STK4 by Western Blot.

Western blot shows lysates of human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MST1/STK4 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MST1/STK4 antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

MST1/STK4 in SH‑SY5Y Human Cell Line.

MST1/STK4 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human MST1/STK4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line

Western Blot

1 µg/mL
Sample: Human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH‑SY5Y human neuroblastoma cell line

Reviewed Applications

Read 4 reviews rated 3.8 using AF4939 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MST1/STK4

MST1 (mammalian sterile twenty-like 1; also Ser/Thr-protein kinase 4) is a 56 kDa class II GC (germinal center) kinase that contains an STE class catalytic domain. It is ubiquitously expressed, and found in both cytoplasm and nucleus. Human MST1 is 487 amino acids (aa) in length. It contains a Ser/Thr protein kinase domain (aa 30‑281), followed by two caspase cleavage sites (Asp326 and 349), two NLS motifs (aa 361‑370 and 441‑451) and a dimerization domain that spans the C‑terminal 57 amino acids. Upon dimerization, MST1 is activated via autophosphorylation at Thr183. Two MST1 variants exist; one shows a substitution of three Lys for the C‑terminal 85 aa, while a second shows a 27 aa substitution for the C‑terminal 55 amino acids. Over aa 285‑443, human MST1 shows 94% aa identity to mouse MST1.

Long Name

Mammalian STE20-like Protein Kinase 1/Serine/Threonine Protein Kinase 4

Alternate Names

KRS2, STK4, YSK3

Entrez Gene IDs

6789 (Human); 58231 (Mouse); 311622 (Rat)

Gene Symbol

STK4

UniProt

Q13043

Additional MST1/STK4 Products

Product Documents for Human MST1/STK4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MST1/STK4 Antibody

For research use only

Citations for Human MST1/STK4 Antibody

Customer Reviews for Human MST1/STK4 Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
75%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 的 4 reviews Showing All
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  • Human MST1/STK4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human MST1/STK4 Antibody AF4939
  • Human MST1/STK4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Human MST1/STK4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human MST1/STK4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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Protocols

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