Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PCPE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PCPE-1
Met1-Asp449
Accession # Q15113

Specificity

Detects human PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PCPE-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human PCPE‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture media from NS0 cells transfected with recombinant human PCPE-1, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human PCPE‑1

Reviewed Applications

Read 1 review rated 4 using AF2627 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PCPE-1

PCPE-1 is a 55 kDa extracellular protein that lacks intrinsic enzymatic activity. It functions as a substrate-specific adaptor protein that stimulates C-terminal processing of the fibrillar procollagens I, II and III by PCP (also known as BMP-1/tolloid metalloproteinases). PCPE is naturally processed into N-terminal fragments of 34 and 36 kDas, both of which also enhance PCP. A 16.5 kDa protein corresponding to a C-terminal fragment of PCPE has been described and shown to have metalloproteinase inhibitor activity. Human PCPE-1 shares approximately 81% amino acid sequence homology with mouse and rat PCPE-1.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Gene Symbol

PCOLCE

UniProt

Q15113

Additional PCPE-1 Products

Product Documents for Human PCPE‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PCPE‑1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human PCPE‑1 Antibody

Customer Reviews for Human PCPE‑1 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human PCPE‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Human Persephin Antibody
    Name: Anonymous
    Application: Protein simple using Jess device.
    Sample Tested: Heart lysates
    Species: Human
    Verified Customer | Posted 09/14/2021
    Saturation of AF2627 antibody signal on heart lysates
    Use antibody at 10 fold dilution (saturation of mAb signal). Protein simple using Jess device, validation of antibody.
    Human PCPE‑1 Antibody AF2627
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies