Human Siglec-8 PE-conjugated Antibody Summary
Met17-Ala363
Accession # Q9NYZ4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 APC-conjugated Monoclonal Antibody (Catalog # FAB155A) and either (A) Mouse Anti-Human Siglec-8 PE-conjugated Monoclonal Antibody (Catalog # FAB7975P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-8
Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares <40% amino acid sequence identity.
Product Datasheets
Citations for Human Siglec-8 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease
Authors: Bavithra Vijayakumar, Karim Boustani, Patricia P. Ogger, Artemis Papadaki, James Tonkin, Christopher M. Orton et al.
Immunity
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Phosphatidylinositol 3-Kinase (PI3K) Orchestrates Aspergillus fumigatus-Induced Eosinophil Activation Independently of Canonical Toll-Like Receptor (TLR)/C-Type-Lectin Receptor (CLR) Signaling
Authors: A Dietschman, S Schruefer, S Westermann, F Henkel, K Castiglion, R Willebrand, J Adam, J Ruland, R Lang, DC Sheppard, J Esser-von-, D Radtke, S Krappmann, D Voehringer
MBio, 2022-06-13;0(0):e0123922.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Siglec-8 antibody reduces eosinophil and mast cell infiltration in a transgenic mouse model of eosinophilic gastroenteritis
Authors: BA Youngblood, EC Brock, J Leung, R Falahati, BS Bochner, HS Rasmussen, K Peterson, C Bebbington, N Tomasevic
JCI Insight, 2019-10-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Siglec-F Promotes IL-33–Induced Cytokine Release from Bone Marrow–Derived Eosinophils Independently of the ITIM and ITIM-like Motif Phosphorylation
Authors: Stefanie Westermann, Axel Dietschmann, Daniela Doehler, Kirstin Castiglione, Bruce S. Bochner, David Voehringer et al.
The Journal of Immunology
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