Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 334703
View all Sortilin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Sortilin
Ser78-Asn755
Accession # Q99523

Specificity

Detects human Sortilin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Sortilin Antibody

Detection of Sortilin antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of Sortilin in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Sortilin Monoclonal Antibody (Catalog # MAB31541, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Sortilin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: K562 Human Cell Line

Reviewed Applications

Read 1 review rated 5 using MAB31541 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sortilin

Sortilin, also known as neurotensin receptor 3, is a 100 kDa transmembrane receptor that is localized both in the endosomal compartment and on plasma membranes. It contains an extracellular/lumenal Vps10p (vacuolar protein-sorting 10 protein) domain and is one of five known members of the mammalian Vps10p-D receptor family. A Sortilin propeptide, cleaved by a furin, blocks premature ligand binding to uncleaved Sortilin and functions as a chaperone to facilitate trafficking in the Golgi compartments. Sortilin binds multiple ligands and has diverse functions. Mature human Sortilin extracellular domain shares 92% and 91% amino acid sequence identity with that of mouse and rat Sortilin, respectively.

Alternate Names

Gp95, Ntr3, SORT1

Entrez Gene IDs

6272 (Human); 20661 (Mouse)

Gene Symbol

SORT1

UniProt

Q99523

Additional Sortilin Products

Product Documents for Human Sortilin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Sortilin Antibody

For research use only

Citations for Human Sortilin Antibody

Customer Reviews for Human Sortilin Antibody (1)

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  • Human Sortilin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/17/2021
    Human Sortilin Antibody MAB31541

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