Human TLR2 Biotinylated Antibody

Catalog #: BAF2616
4 Citations Datasheet / COA / SDS

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BAF2616

All TLR2 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR2 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant mouse TLR2 and less than 0.3% cross-reactivity with recombinant human (rh) TLR3 and rhTLR4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR2
Glu21-Leu590
Accession # O60603

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TLR2 (Catalog # 2616-TR)

Flow Cytometry

2.5 µg/10⁶ cells

Human preipheral blood monocytes

Human TLR2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human TLR2 Antibody (Catalog # AF2616)

Standard: Recombinant Human TLR2 Protein, CF (Catalog # 2616-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

References

Wetzler, L. (2003) Vaccine 21:S2/55.
Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
Akira S. (2003) Curr. Opin. Immunol. 15:5.
Yamamoto M. et al. (2002) Nature 420:324.

Long Name

Toll-like Receptor 2

Entrez Gene IDs

7097 (Human); 24088 (Mouse); 310553 (Rat)

Alternate Names

CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TLR2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation
Authors: C Mayslich, PA Grange, M Castela, AG Marcelin, V Calvez, N Dupin
International Journal of Molecular Sciences, 2022-05-03;23(9):.
Species: Human
Sample Types: Protein
Applications: Western Blot
TLR-2 Recognizes Propionibacterium acnes CAMP Factor 1 from Highly Inflammatory Strains
PLoS ONE, 2016-11-30;11(11):e0167237.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
VB-201, an oxidized phospholipid small molecule, inhibits CD14- and Toll-like receptor-2-dependent innate cell activation and constrains atherosclerosis.
Authors: Mendel I, Feige E, Yacov N, Salem Y, Levi I, Propheta-Meiran O, Shoham A, Ishai E, George J, Harats D, Breitbart E
Clin Exp Immunol, 2014-01-01;175(1):126-37.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Involvement of regulatory T cells in the immunosuppression characteristic of patients with paracoccidioidomycosis.
Authors: Ferreira MC, de Oliveira RT, da Silva RM, Blotta MH, Mamoni RL
Infect. Immun., 2010-07-19;78(10):4392-401.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry