Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Bioassay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1171A
View all CD177 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant mouse CD177
Accession # Q8R2S8

Specificity

Detects mouse CD177 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CD177 Antibody

Detection of CD177 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD177 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rabbit Anti-Mouse CD177 Monoclonal Antibody (Catalog # MAB8186) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Applications for Mouse CD177 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD177

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References

  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
  3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  4. Klippel, S. et al. (2002) Blood 100:2441.
  5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
  6. Temerinac, S. et al. (2000) Blood 95:2569.
  7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
  8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
  9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
  10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
  11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
  12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human); 68891 (Mouse)

Gene Symbol

CD177

UniProt

Q8R2S8

Additional CD177 Products

Product Documents for Mouse CD177 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD177 Antibody

For research use only

Related Research Areas

Neutrophils

Citations for Mouse CD177 Antibody

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