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Rabbit IgG Antibody

Catalog #: AF008
7 Citations Datasheet / COA / SDS
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AF008
R&D Systems Antibodies
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Citations (7)
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Rabbit IgG Antibody Summary

Species Reactivity
Rabbit
Specificity
Detects rabbit IgG heavy and light chains.  In direct ELISAs, less than 5% cross-reactivity with mouse IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease
    Authors: Mansky RH, Greguske EA, Yu D et al.
    Cell reports
  2. Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway
    Authors: N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada
    Journal of dental sciences, 2023;18(2):567-576.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway
    Authors: N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada
    Journal of dental sciences, 2022-09-16;18(2):567-576.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington's Disease and Influences Striatal Synaptic Density
    Authors: N Zarate, TA Intihar, D Yu, J Sawyer, W Tsai, M Syed, L Carlson, R Gomez-Past
    International Journal of Molecular Sciences, 2021-12-04;22(23):.
    Species: Human, Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  5. Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer
    Authors: S Wu, M Luo, KKW To, J Zhang, C Su, H Zhang, S An, F Wang, D Chen, L Fu
    Molecular Cancer, 2021-01-18;20(1):17.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. CXC chemokine receptor 4 signaling upon co-activation with stromal cell-derived factor-1alpha and ubiquitin.
    Authors: Tripathi A, Davis J, Staren D, Volkman B, Majetschak M
    Cytokine, 2013-12-24;65(2):121-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
    Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
    Arthritis Rheum., 2006-03-01;54(3):765-78.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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