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Goat IgG HRP-conjugated Antibody

R&D Systems | Catalog # HAF017

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Goat

Applications

Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Goat IgG

Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Goat IgG HRP-conjugated Antibody

Detection of Goat IgG Primary Antibody by Western Blot

Detection of Goat IgG Primary Antibody by Western Blot

Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin 1A Polyclonal Antibody (Catalog # AF7237) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IgG by Simple Western

Detection of Human IgG by Simple Western

Evaluation of ZL-2201 antiproliferative activity in vitro. A, Chemical structure of ZL-2201, a potent and selective DNA-PK inhibitor. B, Concentration-dependent response to ZL-2201 in M059J and M059K glioblastoma cancer cells measured by CTG after 6-day of treatment. The graph represents the average inhibitory (IC50) values (n = 3). C, Concentration-dependent response to ZL-2201 in CRISPR KO of ATM in A549 and FaDu cancer cells measured by CTG after 6 days of treatment. The graph represents the average inhibitory (IC50) values (n = 2–5). D, IC50 responses to ZL-2201 in ATM mutant cell lines (black bars) versus ATM WT A549 cell line (gray bar). Cell growth was measured by CTG after 6 days of treatment. The graph represents the average inhibitory (IC50) values (n = 2–6). NCI-H1703 (E) and A549 (F) cancer cells were treated with bleomycin (10 μmol/L) for 2 hours followed by the addition of increasing concentration of ZL-2201 for 2 hours. Whole-cell lysates were harvested, and concentration-dependent inhibition of DNA-PK protein were analyzed by Simple Western. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37663435), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IgG by Simple Western

Detection of Human IgG by Simple Western

Evaluation of ZL-2201 antiproliferative activity in vitro. A, Chemical structure of ZL-2201, a potent and selective DNA-PK inhibitor. B, Concentration-dependent response to ZL-2201 in M059J and M059K glioblastoma cancer cells measured by CTG after 6-day of treatment. The graph represents the average inhibitory (IC50) values (n = 3). C, Concentration-dependent response to ZL-2201 in CRISPR KO of ATM in A549 and FaDu cancer cells measured by CTG after 6 days of treatment. The graph represents the average inhibitory (IC50) values (n = 2–5). D, IC50 responses to ZL-2201 in ATM mutant cell lines (black bars) versus ATM WT A549 cell line (gray bar). Cell growth was measured by CTG after 6 days of treatment. The graph represents the average inhibitory (IC50) values (n = 2–6). NCI-H1703 (E) and A549 (F) cancer cells were treated with bleomycin (10 μmol/L) for 2 hours followed by the addition of increasing concentration of ZL-2201 for 2 hours. Whole-cell lysates were harvested, and concentration-dependent inhibition of DNA-PK protein were analyzed by Simple Western. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37663435), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IgG by Simple Western

Detection of Human IgG by Simple Western

Phenotypic evaluation of ZL-2201 synergy with various DNA-damaging agents. C, NCI-H1703 cells were treated with ZL-2201 (0.1, 0.3, 0.5, 1, and 3.6 μmol/L) and a low dose of doxorubicin (0.006 μmol/L) for 2 hours. Whole-cell lysates were harvested, and Simple Western was used to show the inhibition of pDNA-PK upon combination treatment of ZL-2201 with doxorubicin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37663435), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Goat IgG HRP-conjugated Antibody

Application
Recommended Usage

Western Blot

Use at a 1:1000 dilution.

Reviewed Applications

Read 15 reviews rated 4.9 using HAF017 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG HRP-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Goat IgG HRP-conjugated Antibody

For research use only

Citations for Goat IgG HRP-conjugated Antibody

Customer Reviews for Goat IgG HRP-conjugated Antibody (15)

4.9 out of 5
15 Customer Ratings
5 Stars
93%
4 Stars
7%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 15 reviews Showing All
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  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipocytes
    Species: Human
    Verified Customer | Posted 07/17/2025
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/10/2025
    Otx2 ab identified by Goat IgG HRP conjugated ab
    Goat IgG HRP-conjugated Antibody HAF017
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Works great
    Name: Anonymous
    Application: Western Blot
    Sample Tested: in vitro cell culture
    Species: Human
    Verified Customer | Posted 09/09/2024
    Dot blot showing compatibility of a primary antibody with HAF017
    This secondary antibody has always worked in my assays (WB, ELISA and dot blot)
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Sampath Kumar Anandan
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Purified recombinant protein
    Verified Customer | Posted 04/15/2024
    I had loaded 1μg of recombinant myostatin/GDF8 into a 4-15% gel, performed electrophoresis with MES buffer and blotted the gel onto 0.2μm nitrocellulose membrane. For the immuno-detection, I had used the R&D systems goat polyclonal GDF8 antibody (BAF788) at 0.1 or 0.25 µg/mL concentrations, and the R&D system anti-goat secondary antibody (HAF017) at 1:4000 dilution.
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: mouse serum and Mouse
    Verified Customer | Posted 10/23/2023
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 08/31/2023
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mv1Lu mink lung epithelial cell line
    Species: Mink
    Verified Customer | Posted 11/20/2019
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/07/2019
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: human liver cell, Muscle tissue, Skin tissue and Liver tissue
    Species: Human and Pig
    Verified Customer | Posted 08/14/2019
    Dot blot test: The protein samples were neat and not diluted. The samples were compared against three control dilutions from human liver cells (neat, 1:2, and 1:5) which were also targeted for GAPDH.
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/20/2019
  • Goat IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: ELISA
    Sample Tested: Serum
    Species: Goat
    Verified Customer | Posted 05/01/2018
  • Goat IgG HRP-conjugated Antibody
    Name: Adrie Jones
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 10/23/2017
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/24/2017
    Western blot analysis of Human PD-1 in PBMCs using Goat anti-Human PD-1 antibody followed by HRP-conjugated Rabbit anti-Goat secondary antibody (#HAF017) at 1:1,000 dilution in 3% BSA-PBS blocking buffer.
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue and Heart tissue
    Species: Mouse
    Verified Customer | Posted 08/26/2016
    Goat IgG HRP-conjugated Antibody HAF017
  • Goat IgG Horseradish Peroxidase-conjugated Antibody
    Name: Yinan Zhang
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Species: Rabbit
    Verified Customer | Posted 02/22/2016
    The HRP-conjugated antibody worked as it is supposed to be. No complain. Will buy the product in the future.

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