Key Product Details

Species Reactivity

Goat

Applications

Western Blot, Simple Western

Label

HRP

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Goat IgG

Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Goat IgG HRP-conjugated Antibody

Detection of Goat IgG Primary Antibody by Western Blot

Detection of Goat IgG Primary Antibody by Western Blot

Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Polyclonal Antibody (Catalog # AF3584) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Goat IgG Primary Antibody by Simple WesternTM.

Detection of Goat IgG Primary Antibody by Simple WesternTM.

Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog # AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Goat IgG HRP-conjugated Antibody

Application
Recommended Usage

Simple Western

Use at a 1:50 dilution.

Western Blot

Use at a 1:1000 dilution.

Reviewed Applications

Read 4 reviews rated 4 using HAF109 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 um filtered solution in PBS with stabilizers.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG HRP-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Goat IgG HRP-conjugated Antibody

For research use only

Citations for Goat IgG HRP-conjugated Antibody

Customer Reviews for Goat IgG HRP-conjugated Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 10/11/2023
    Goat IgG HRP-conjugated Antibody HAF109
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein, bEnd.3 mouse endothelioma cell line and Brain (preoptic nucleus)
    Species: Mouse
    Verified Customer | Posted 11/27/2020
  • Goat IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: EDTA Plasma
    Species: Cynomolgus Monkey
    Verified Customer | Posted 05/01/2020
    used as a secondary antibody on Simple Western
    Goat IgG HRP-conjugated Antibody HAF109
  • Goat IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 05/01/2018

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FAQs for Goat IgG HRP-conjugated Antibody

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  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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