Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

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IC002R
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Mouse IgG1 Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Improving Cancer Targeting: A Study on the Effect of Dual-Ligand Density on Targeting of Cells Having Differential Expression of Target Biomarkers
    Authors: Nayer Sultana, Allan E. David
    International Journal of Molecular Sciences
  2. Oncogenic BRAF induces whole-genome doubling through suppression of cytokinesis
    Authors: R Darp, MA Vittoria, NJ Ganem, CJ Ceol
    Oncogene, 2022-07-15;13(1):4109.
    Species: Zebrafish
    Sample Types: Whole Cells
    Applications: ICC
  3. Peripheral blood brain-derived neurotrophic factor level and tyrosine kinase B expression on T lymphocytes in systemic lupus erythematosus: Implications for systemic involvement
    Authors: B Tian, C Yang, J Wang, X Hou, S Zhao, Y Li, P Yang
    Cytokine, 2019-06-27;123(0):154764.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Association of C-type lectin 18 levels with extrahepatic manifestations in chronic HCV infection
    Authors: TL Liao, YL Huang, YM Chen, HC Lee, DY Chen, SL Hsieh
    Sci Rep, 2018-11-23;8(1):17287.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Coagulation potential and the integrated omics of extracellular vesicles from COVID-19 positive patient plasma
    Authors: S Setua, K Thangaraju, M Dzieciatko, RB Wilkerson, T Nemkov, DR Lamb, Y Tagaya, T Boyer, T Rowden, A Doctor, A D'Alessand, PW Buehler
    Scientific Reports, 2022-12-23;12(1):22191.

FAQs

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Reviews for Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

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Mouse IgG1 Alexa Fluor® 647-conjugated Antibody
By Anonymous on 11/08/2021
Application: Flow Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse