Mouse/Rat Integrin beta 1/CD29 Antibody

Catalog #: AF2405
18 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2405
AF2405-SP

Detection of Mouse and Rat Integrin beta 1/CD29 by Western Blot.

3 Images

Product Details

Citations (18)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Integrin beta 1/CD29 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1
Gln21-Ala738
Accession # P09055

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

B16‑F10 mouse melanoma cell line

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse and Rat Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-3 human prostate cancer cell line, Rat-2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western

View Larger

Detection of Mouse and Rat Integrin beta 1/CD29 by Simple Western^TM. Simple Western lane view shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Alternate Names

CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat Integrin beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
Filter your results:

Filter by:

SCA-1/Ly6A Mesodermal Skeletal Progenitor Subpopulations Reveal Differential Commitment of Early Limb Bud Cells
Authors: Jessica Cristina Marín-Llera, Carlos Ignacio Lorda-Diez, Juan Mario Hurle, Jesús Chimal-Monroy
Frontiers in Cell and Developmental Biology
RNF213 gene silencing upregulates transforming growth factor beta 1 expression in bone marrow‑derived mesenchymal stem cells and is involved in the onset of Moyamoya disease
Authors: Changshui Wang, Cuilian Sun, Yueshu Zhao, Huimin Song, Zhengyou Li, Feng Jin et al.
Experimental and Therapeutic Medicine
Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagen
Authors: Chao Liu, Jiao Sun
Experimental and Therapeutic Medicine
Tissue Inhibitor of Metalloproteinases-1 Overexpression Mediates Chemoresistance in Triple-Negative Breast Cancer Cells
Authors: Agnello, L;d'Argenio, A;Caliendo, A;Nilo, R;Zannetti, A;Fedele, M;Camorani, S;Cerchia, L;
Cells
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth
Authors: Y Xue, C Lyu, A Taylor, A Van Ee, A Kiemen, Y Choi, N Khavanian, D Henn, C Lee, L Hwang, E Wier, S Wang, S Lee, A Li, C Kirby, G Wang, PH Wu, D Wirtz, LA Garza, SK Reddy
Science Advances, 2022-04-27;8(17):eabl8698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IF
Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis
Authors: A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco
Cell Reports, 2021-10-26;37(4):109885.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
Species: Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis
Authors: A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco
Cell Rep, 2020-04-14;31(2):107513.
Species: Mouse
Sample Types: Testes
Applications: IF
Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
Nat. Cell Biol, 2017-02-20;19(3):189-201.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
Authors: Wu, B, Cloer, C, Lu, P, Milazi, S, Shaban, M, Shah, S N, Marston-Poe, L, Moulton, H M, Lu, Q L
Gene Ther, 2014-06-19;21(9):785-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
J. Neurochem., 2011-01-19;118(4):521-32.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation

Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
Authors: Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A
Arthritis Rheum., 2009-09-01;60(9):2817-21.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot

Galectin-7 in the control of epidermal homeostasis after injury.
Authors: Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F
Mol. Biol. Cell, 2008-10-01;19(12):5541-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Authors: Chang Y, Finnemann SC
J. Cell. Sci., 2007-08-07;120(0):3053-63.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation

The spatiotemporal expression patterns of MSC-associated markers contribute to the identification of progenitor subpopulations in developing limbs
Authors: Argelia S. García-Cervera, Jesús Chimal-Monroy, Jessica C. Marín-llera
The International Journal of Developmental Biology

Periostin and matrix stiffness combine to regulate myofibroblast differentiation and fibronectin synthesis during palatal healing
Authors: Georgia Nikoloudaki, Paige Snider, Olga Simmons, Simon. J. Conway, Douglas W. Hamilton
Matrix Biology

Proteomics reveals Rictor as a noncanonical TGF-beta signaling target during aneurysm progression in Marfan mice
Authors: Sarah J. Parker, Aleksandr Stotland, Elena MacFarlane, Nicole Wilson, Amanda Orosco, Vidya Venkatraman et al.
American Journal of Physiology-Heart and Circulatory Physiology

Endothelial semaphorin 7A promotes seawater aspiration-induced acute lung injury through plexin C1 and beta 1 integrin
Authors: Minlong Zhang, Xue Yan, Wei Liu, Ruilin Sun, Yonghong Xie, Faguang Jin
Molecular Medicine Reports