Porcine IL-6 Antibody Summary
Pro29-Met212
Accession # P26893
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑6 in Porcine PBMCs. IL-6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6
Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).
- Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
- Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
- Jones, S.A. (2005) J. Immunol. 175:3468.
- Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
- Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
- Murakami, M. et al. (1993) Science 260:1808.
- Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
- Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Product Datasheets
Citations for Porcine IL-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
J. Immunol., 2017-06-19;0(0):.
Species: Porcine
Sample Types: Whole Cells
Applications: Functional Assay -
Altered expression of cytokines IL-1?, IL-6, IL-8 and TNF-? in bovine follicular persistence
Authors: AF Stassi, ME Baravalle, EM Belotti, F Rey, NC Gareis, PU Díaz, FM Rodríguez, CJ Leiva, HH Ortega, NR Salvetti
Theriogenology, 2017-04-26;97(0):104-112.
Species: Bovine
Sample Types: Whole Tissue
Applications: IHC -
Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant
Authors: C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi
PLoS ONE, 2017-01-20;12(1):e0170504.
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry -
omega -3 PUFA Rich Camelina Oil By-Products Improve the Systemic Metabolism and Spleen Cell Functions in Fattening Pigs
Authors: Ionelia Taranu, Mihail Gras, Gina Cecilia Pistol, Monica Motiu, Daniela E. Marin, Nicoleta Lefter et al.
PLoS ONE
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Seminal plasma did not influence the presence of transforming growth factor-beta 1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination
Authors: Jiwakanon Jatesada, Persson Elisabeth, Dalin Anne-Marie
Acta Veterinaria Scandinavica
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Mutations in classical swine fever virus NS4B affect virulence in swine.
Authors: Fernandez-Sainz I, Gladue DP, Holinka LG
J. Virol., 2009-11-18;84(3):1536-49.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-Fr -
Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
Authors: Gossl M, Versari D, Lerman LO, Chade AR, Beighley PE, Erbel R, Ritman EL
Atherosclerosis, 2009-03-25;206(2):362-8.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC -
Sex difference in link between interleukin-6 and stress.
Authors: Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
Endocrinology, 2007-05-17;148(8):3758-64.
Species: Porcine
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
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